Thermo Scientific™ ThermoPrime Taq DNA Polymerase, with 10x buffer and separate vial of 25 mM MgCl₂

For PCR applications requiring increased yield and sensitivity, these PCR kits and master mixes are optimized to produce large amounts of PCR product.

Supplier:  Thermo Scientific™ AB0301B

Catalog No. AB-0301B

Description

Thermo Scientific™ ThermoPrime mixes are optimized for PCR applications requiring increased yield and sensitivity, to produce large amounts of PCR product.

ThermoPrime Taq DNA Polymerase is a 94 kDa recombinant thermostable DNA polymerase obtained by high level expression of the Taq DNA polymerase gene in E. coli. A proprietary buffer component protects ThermoPrime during high temperature steps, ensuring optimal performance throughout the reaction, leading to increased PCR yields. Convenient Mater Mix format is also available for easier reaction set-up.

Highlights:

• Enhanced enzyme stability for increased yield
• Advanced buffering system minimizes PCR optimization steps
• Convenient Master Mix formats save time and reduce the risk of sample contamination during assay setup

Recommended for:

Amplifying fragments up to 5kb; Routine PCR

Specifications

• Content And Storage: Contains:
  • 10 x 50 µL ThermoPrime Taq DNA Polymerase (5 U/µL)
  • 10 x 1.5 mL 10X Reaction Buffer IV
  • 10 x 1.5 mL 25 mM MgCl₂
  
  Store at -20°C.
• GC-Rich PCR Performance: Low
• Reaction Speed: Standard
• Product Type: DNA Polymerase
• Concentration: 10X
• Hot Start: No
• Overhang: 3'-A
• Starting Material: DNA

• Detection Method: Primer-probe
• Polymerase: ThermoPrime Taq DNA Polymerase
• Product Line: ThermoPrime
• Quantity: 4000 x 25 μL Reactions
• Fidelity (vs. Taq): 1 X
• No. of Reactions: 4000 Reactions
• Reaction Format: Standalone

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.