Thermo Scientific™ Phusion Green High-Fidelity DNA Polymerase (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR.

Includes:

Phusion High-Fidelity DNA Polymerase

• Phusion DNA Polymerase 2U/μL
• 5x Phusion HF Buffer
• 5x Phusion GC Buffer
• DMSO
• 50 mM MgCl₂ solution

Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl₂ in the final 1x concentration.

Phusion Green High-Fidelity DNA Polymerase

• Phusion DNA Polymerase 2U/μL
• 5x Phusion Green HF Buffer
• 5x Phusion Green GC Buffer
• DMSO
• 50 mM MgCl₂ solution

Both Phusion Green HF Buffer and Phusion Green GC Buffer provide 1.5 mM MgCl₂ in the final 1x concentration.

Catalog Number	Mfr. No.	No. of Reactions
F534S	Thermo Scientific™ F534S	100 Reactions
F534L	Thermo Scientific™ F534L	500 Reactions

Description

Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.

The Phusion Green format is a combination of Phusion High-Fidelity DNA Polymerase with 5X Phusion Green Buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The density reagent and dyes do not interfere with excellent performance of Phusion DNA Polymerases and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Highlights
• High fidelity (52X Taq)
• Fast PCR due to short extension times (15-30 s/kb)
• Robust performance, minimal optimization needed
• High yields of PCR products with minimal enzyme amounts
• Available as a Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)

Applications
• High-fidelity PCR
• Cloning
• Template generation for sequencing
• Amplification of difficult (GC-rich) templates
• Long-range PCR (up to 20 kb)
• Mutagenesis
• High throughput PCR
• Microarray

Using Phusion DNA Polymerases
Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.

Specifications

• Content And Storage: • Phusion DNA Polymerase, 2 U/μL
  • 5X Phusion Green HF and GF Buffers
  • DMSO
  • 50 mM MgCl₂
  
  Phusion Green HF and Phusion Green GC Buffers each provide 1.5 mM MgCl₂ in the final 1X concentration.
  Store at -20°C.
• Polymerase: Phusion High-Fidelity DNA Polymerase
• Product Type: High-Fidelity DNA Polymerase
• For Use With (Application): Standard PCR, High-fidelity PCR
• Fidelity (vs. Taq): 52X
• Overhang: Blunt
• Size (Final Product): 20 kb or less

• GC-Rich PCR Performance: High
• Reaction Speed: Fast
• Shipping Condition: Dry Ice
• Concentration: 2 U/μL
• Hot Start: No
• Reaction Format: Standalone

For Research Use Only. Not for use in diagnostic procedures.