Thermo Scientific™ Phusion U Hot Start PCR Master Mix

Thermo Scientific Phusion U DNA Polymerase is a proofreading enzyme (25x Taq) with a proprietary mutation to read through uracils and incorporate dUTP

Catalog Number	Mfr. No.	Quantity
F533S	Thermo Scientific™ F533S	100 x 50 μL Reactions
F533L	Thermo Scientific™ F533L	500 x 50 μL Reactions

Description

Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology. Due to a proprietary mutation in the so called dUTP binding pocket of Phusion, Phusion U overcomes an important limitation of proofreading enzymes - it is able to incorporate dUTP and read through uracil present in DNA templates.
In addition to uracil usage possibility, Phusion U features all the superior properties of other Phusion DNA Polymerases - great accuracy, speed, ability to amplify long amplicons up to 20 kb and a high specificity with Affibody based hot start . These features make Phusion U Hot Start DNA Polymerase an excellent choice for such important applications as amplification of bisulphite-converted or damaged DNA as well as use of carryover contamination control.
Phusion U Hot Start DNA Polymerase is also available in the Master Mix and Green formats. Phusion U Hot Start PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. Only template and primers need to be added by the user. Phusion U Green format is a combination of Phusion U Hot Start DNA Polymerase and 5X Phusion Green Buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with Phusion U performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Highlights

• Accuracy - extremely high fidelity DNA polymerase (25x Taq)
• Uracil-tolerance - engineered to incorporate dUTP and amplify uracil containing templates
• Specificity - hot start for reduced nonspecific amplification and primer degradation.
• Speed - short extension times (15-30 s/kb)
• Direct loading of PCR products on gels

Applications

• Amplification of bisulphite-converted DNA
• Amplification of damaged or aged DNA
• Carry-over contamination control
• Uracil-excision based (USER) cloning methods

Includes:
Phusion U Hot Start DNA Polymerase (F-555)

• Phusion U Hot Start DNA Polymerase, 2 U/μL
• 5X Phusion HF Buffer
• 5X Phusion GC Buffer
• DMSO

Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl₂ in the final 1X concentration.

Phusion U Green Hot Start DNA Polymerase (F-556)

• Phusion U Hot Start DNA Polymerase, 2 U/μL
• 5X Phusion Green HF Buffer
• 5X Phusion Green GC Buffer
• DMSO

Both Phusion Green HF and Phusion Green GC Buffer provide 1.5 mM MgCl₂ in the final 1X concentration.

Phusion U Hot Start PCR Master Mix

• 2X Phusion U PCR Master Mix (Phusion U Hot Start DNA Polymerase, 2X reaction buffer, 3 mM MgCl₂, 400 μM of each dATP, dTTP, dCTP, and dGTP)
• Water, nuclease-free

Hazardous:No
Storage buffer:20 mM Tris-HCl (pH 7.4 at 25°C), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, stabilizers, and 50 % glycerol.
Storage Condition:-20°C

Specifications

• Content And Storage: Phusion U Hot Start PCR Master Mix includes:
  • Phusion U Hot Start DNA Polymerase
  • 2X reaction buffer
  • 3 mM MgCl₂
  • 400 µM of each dATP, dTTP, dCTP and dGTP
  The master mix is supplied with nuclease-free water.
  Store at -20°C.
  
• GC-Rich PCR Performance: Medium
• Reaction Speed: Fast
• Product Type: Hot Start PCR Master Mix
• Fidelity (vs. Taq): 25 X
• Overhang: Blunt
• Size (Final Product): 20 kb

• Format: Tube
• Polymerase: Phusion U Hot Start DNA Polymerase
• Product Line: Phusion
• Concentration: 2X
• Hot Start: Built-In Hot Start
• Reaction Format: SuperMix or Master Mix
• Starting Material: DNA