Thermo Scientific™ Phire Tissue Direct PCR Master Mix

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.

Supplier:  Thermo Scientific™ F170S

Catalog No. F170S

Description

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects. The Master Mix containing Phire Hot Start II DNA Polymerase is specially formulated to perform PCR in the presence of different animal tissue-derived inhibitors such as collagen, melanin and eumelanin (hair, skin) or myoglobin (muscle). The kit also includes Thermo Scientific DNARelease Additive, which can be used to improve the release of DNA from difficult tissues.

Highlights

• Direct PCR—sample is added directly to PCR reaction, therefore there is no need for time-consuming and expensive DNA purification steps
• Dilution & Storage protocol available—allows tens of PCR reactions from one tiny sample and allows re-testing
• Specially engineered Phire DNA polymerase—extremely short PCR protocol times
• Master Mix format with premixed gel loading dye—minimizes possibility of cross-contamination, reduces sample handling and allows direct loading on gel

Applications

• Genotyping
• Transgene detection
• Knockout analysis
• Sequencing

The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.

For optimal results start by accurately calculating your Tm with our Tm calculator.

Specifications

• Content And Storage: • 2X Phire Tissue Direct PCR Master Mix, 2 x 1.25 mL
  • Dilution Buffer, 5 mL
  • DNARelease Additive, 3 x 100 μL
  • 25 μM Control Primer Mix, 40 μL
  • Nuclease-free water, 2 x 1.25 mL
  • 50 μg O'GeneRuler Express DNA Ladder
  
  Store at -20°C.
• PCR Method: Direct PCR
• Reaction Speed: Fast
• Product Type: Direct PCR Master Mix
• For Use With (Application): Genotyping, Hot-start PCR
• Hot Start: Built-In Hot Start
• Overhang: Blunt
• Sample Type: Tissue

• GC-Rich PCR Performance: Medium
• Polymerase: Phire Hot Start II DNA Polymerase
• Includes Label or Dye: Yes
• Shipping Condition: Wet or Dry Ice
• Concentration: 2X
• No. of Reactions: 250 Reactions
• Reaction Format: SuperMix or Master Mix
• Starting Material: DNA

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.