Thermo Scientific™ Phire Green Reaction Buffer

The reaction buffer for Thermo Scientific Phire Hot Start II DNA Polymerase is available in three formats: non-colored version with and without detergents or Green version containing a density reagent and two tracking dyes for direct loading of PCR products on gel.

Supplier:  Thermo Scientific™ F527L

Catalog No. F527L

Includes: Phire Green Reaction Buffer

• 5x Phire Green Reaction Buffer (1X buffer provides 1.5 mM MgCl₂)
• 50 mM MgCl₂ solution
• DMSO

Description

The reaction buffer for Thermo Scientific Phire Hot Start II DNA Polymerase is available in three formats: non-colored version with and without detergents (Cat. No. F-524L and F-525L, respectively) or Green version (F-527L) containing a density reagent and two tracking dyes for direct loading of PCR products on gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

The concentration of Mg²⁺ in the reaction buffers is optimized to work well for most amplicons. The buffer contains 1.5 mM MgCl₂ at final reaction concentration. Separate tubes of MgCl₂ and DMSO are provided for further optimization.

Related Products

• Phire Reaction Buffer (Cat. No. F524L)
• Phire Reaction Buffer, detergent-free (F525L)

Specifications

• Content And Storage: • 5X Phire Green Reaction Buffer, 4 x 1.5 mL
  • 50 mM MgCl₂, 2 x 1.5 mL
  • DMSO, 0.5 mL
  
  Store at -20°C.
• Product Type: PCR Reaction Buffer
• Shipping Condition: Wet or Dry Ice
• Concentration: 5X

• Polymerase: Phire Hot Start II DNA Polymerase
• Quantity: 4 x 1.5 mL
• For Use With (Application): Hot-start PCR

For Research Use Only. Not for use in diagnostic procedures.