Invitrogen™ Quant-iT™ OliGreen™ ssDNA Assay Kit and Quant-iT OliGreen ssDNA Reagent

Quant-iT™ OliGreen ssDNA Assay Kit and Quant-iT OliGreen ssDNA Reagent contain an ultrasensitive, green-fluorescent nucleic acid stain for quantitating oligonucleotides and single-stranded DNA (ssDNA) in solution.

Catalog Number	Mfr. No.	Product Type	Quantitation Range
O11492	Invitrogen™ O11492	Quant-iT OliGreen ssDNA Kit	200 pg to 2 μg
O7582	Invitrogen™ O7582	Quant-iT OliGreen ssDNA Reagent	200 pg to 2 μg

Description

Quantify ssDNA with ease using the Quant-iT OliGreen ssDNA Assay kit and reagent. This assay kit and reagent enable sensitive quantitation of single-stranded DNA without the need for a highly concentrated sample. As little as 100 pg/mL can be quantitated using the assay kit or Quant-iT OliGreen ssDNA Reagent.

Quantitate ssDNA and other oligonucleotides accurately and without the need for a highly concentrated sample with Quant-iT OliGreen ssDNA Assay Kit and Quant-iT OliGreen ssDNA Reagent. Quant-iT OliGreen ssDNA Assay Kits contain an ultrasensitive, green-fluorescent nucleic acid stain for quantitating oligonucleotides and single-stranded DNA (ssDNA) in solution. The Quant-iT™ OliGreen™ ssDNA Assay Reagent enables researchers to quantify as little as 1 ng/mL oligonucleotide or ssDNA (200 pg in a 200 μL assay volume) with a fluorescence microplate reader using fluorescein excitation and emission wavelengths. This sensitivity exceeds that achieved with absorbance methods by 10,000-fold.

In addition to the dye, the OliGreen ssDNA Assay Kit includes buffer and an oligonucleotide standard. The OliGreen reagent is also available as the stand-alone Quant-iT OliGreen ssDNA Reagent.

Specifications

• Detection Method: Fluorescence
• For Use With (Equipment): Microplate Reader
• Quantitation Range: 200 pg to 2 μg
• Shipping Condition: Room Temperature

• Excitation/Emission: 500/525
• No. of Reactions: 200 assays (2 mL assay volume)
• Product Line: OLIGREEN, Quant-iT