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Promega Apo-ONE™ Homogeneous Caspase-3/7 Assay Kit
Highly sensitive, homogeneous fluorescent apoptosis assay for use in cultured cells and with purified caspase. Just add-mix-read.
Supplier: Promega ZG7791
Includes: Apo-ONE Caspase Substrate Z-DEVD-R110, 100X; Apo-ONE Homogeneous Caspase Buffer
Description
Substrate and buffer solutions are combined to make the Apo-ONE Caspase-3/7 Reagent that is added directly to samples. The amount of fluorescent product generated is representative of the amount of active Caspase-3/7 present in the sample.
- May be used with purified enzyme preparations, cell extracts, or cultures of adherent, suspension, or primary cells; compatible with use in cuvets and 96- or 384-well plates
- Simple “add, mix, and measure” protocol and high assay sensitivity decreases time to result; requires fewer cells than comparable caspase assays
- Single reagent addition format is compatible with high-throughput applications
- Apo-ONE Homogeneous Caspase-3/7 Buffer is also available separately
Specifications
| 10 x 100 mL | |
| 1 x 1mL 100X Caspase substrate Z-DEVD-R110, 1 x 100mL Apo-ONE™ Homogeneous Caspase-3/7 Buffer | |
| Fluorescence |
| Cuvets, 96- or 384-well Plates | |
| -20°C |
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