Invitrogen™ Platinum™ Taq DNA Polymerase, DNA-free

For low-biomass microbiome studies requiring the highest sensitivity without false-positive results from reagent-borne contamination. Manufactured with stringent quality-control testing to minimize the risk of contamination with bacterial and human DNA.

Supplier:  Invitrogen™ 15966005

Catalog No. 15-966-005

Description

• Certified low-DNA contamination level (≤0.01 copy of bacterial gDNA per enzyme unit)
• Confidence in qualitative and quantitative detection of low-abundance microorganisms due to signal absence with no-template/reagents-only PCR controls
• Same performance as Platinum Taq DNA Polymerase manufactured using conventional process

Platinum Taq DNA Polymerase, DNA-free, is a recombinant Taq polymerase complexed with a proprietary antibody that blocks polymerase activity at ambient temperatures and dissociates after the initial denaturation step at 94°C. This automatic hot start provides increased sensitivity, specificity, and yield, while allowing convenient reaction assembly at room temperature. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase, DNA-free, has a non-template-dependent terminal transferase activity that adds a 3′ deoxyadenosine to product ends, and has a 5′ to 3′ exonuclease activity.

• For the amplification of DNA from complex genomic and viral templates, as well as in RT-PCR
• Ideal for PCR assays where higher sensitivity and specificity are needed to minimize ambiguous or false-positive results

Specifications

• Content And Storage: • Platinum Taq DNA Polymerase, DNA-free, 100 μL
  • 10X PCR Buffer (-MgCl₂), DNA-free, 1.25 mL
  • 50 mM MgCl₂, DNA-free, 1 mL
  
  Store at -5 to -30°C in a non-frost-free freezer.
  
  
• GC-Rich PCR Performance: Low
• Reaction Speed: Standard
• Product Type: Taq DNA Polymerase
• Shipping Condition: Dry Ice
• Hot Start: Built-In Hot Start
• Overhang: 3'-A
• Size (Final Product): 5 kb or Less

• Format: Tube
• Polymerase: Platinum Taq DNA Polymerase
• Product Line: Platinum™
• Quantity: 500 U
• Fidelity (vs. Taq): 1 X
• No. of Reactions: 400 Reactions of 25 μL
• Reaction Format: Standalone
• Starting Material: DNA

For Research Use Only. Not for use in diagnostic procedures.