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Invitrogen™ Platinum™ SuperFi™ DNA Polymerase
Invitrogen Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR.
Supplier: Invitrogen™ 12351250
Description
Invitrogen Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR. Featuring >300X Taq fidelity, Platinum SuperFi DNA Polymerase is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy.
Platinum SuperFi DNA Polymerase is engineered with a DNA-binding domain resulting in high processivity and increased resistance to PCR inhibitors. This feature also enables fast-cycling protocols and amplification of long targets. The Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation.
Benefits of Platinum SuperFi DNA Polymerase
- Exceptional >300X Taq fidelity
- High specificity and increased yields with Platinum hot-start technology
- Robust amplification of difficult targets including those of sub-optimal purity or high GC content
- Room temperature reaction setup and 24 hour bench top stability of pre-assembled reactions
- Amplification of samples with suboptimal purity
Applications
- High-fidelity PCR
- Cloning and sub-cloning
- Site-directed mutagenesis
- Amplification of GC-rich templates
- Template generation for sequencing
- High-throughput PCR
- Long PCR
Notes
- Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).
Specifications
5 boxes of Platinum SuperFi Master Mix, each containing: • Platinum SuperFi DNA Polymerase (250 μL at 2 U/μL) • 5X SuperFi Buffer (6 x 1.25 mL) • 5X SuperFi GC Enhancer (4 x 1.25 mL) Store at –5°C to –30°C. |
|
Platinum SuperFi DNA Polymerase | |
Wet or Dry Ice | |
300X | |
5 x 500 Reactions | |
Separate Components |
High | |
Fast | |
Hot-start PCR, High-fidelity PCR | |
Built-In Hot Start | |
Blunt | |
20 kb or less |
Safety and Handling
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For Research Use Only. Not for use in diagnostic procedures.