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Invitrogen™ Platinum™ SuperFi™ DNA Polymerase

Invitrogen Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR.

Supplier:  Invitrogen™ 12351050

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Catalog No. 12-351-050


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Description

Description

Invitrogen Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR. Featuring >300X Taq fidelity, Platinum SuperFi DNA Polymerase is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy.

Platinum SuperFi DNA Polymerase is engineered with a DNA-binding domain resulting in high processivity and increased resistance to PCR inhibitors. This feature also enables fast-cycling protocols and amplification of long targets. The Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation.

Benefits of Platinum SuperFi DNA Polymerase

  • Exceptional >300X Taq fidelity
  • High specificity and increased yields with Platinum hot-start technology
  • Robust amplification of difficult targets including those of sub-optimal purity or high GC content
  • Room temperature reaction setup and 24 hour bench top stability of pre-assembled reactions
  • Amplification of samples with suboptimal purity

Applications

  • High-fidelity PCR
  • Cloning and sub-cloning
  • Site-directed mutagenesis
  • Amplification of GC-rich templates
  • Template generation for sequencing
  • High-throughput PCR
  • Long PCR

Notes

  • Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).
TRUSTED_SUSTAINABILITY
Specifications

Specifications

• Platinum SuperFi DNA Polymerase (250 μL at 2 U/μL)
• 5X SuperFi Buffer (6 x 1.25 mL)
• 5X SuperFi GC Enhancer (4 x 1.25 mL)

Store at –5°C to –30°C.
Platinum SuperFi DNA Polymerase
Wet or Dry Ice
300X
500 Reactions
Separate Components
High
Fast
Hot-start PCR, High-fidelity PCR
Built-In Hot Start
Blunt
20 kb or less
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