Thermo Scientific™ Pierce™ Centrifuge Columns, 0.8 mL

Pack these empty 0.5 to 10 mL spin columns with agarose beads to perform small-scale affinity purification methods in 15- or 50-mL conical microcentrifuge tubes.

Supplier:  Thermo Scientific™ 89869

Catalog No. PI89869

Description

Thermo Scientific Pierce Centrifuge Columns are convenient tools for handling 40 μL to 10 mL of an affinity support for purification.

Column Details

• Made from low protein binding polypropylene for compatibility with protein purification
• Total volume: 800 μL
• Resin volume: 40-400 μL
• Filter type: Polyethylene filter, ∼30 μm pore size
• Receiver tube: Fits standard microcentrifuge tubes
• Caps: O-ring screw-top cap
• Twist-off bottom

Simply add the affinity resin to one of the polypropylene columns, remove the twist-off bottom and allow the resin to pack itself. Then add your sample and allow it to bind to the support and use a centrifuge to efficiently wash away any contaminants and elute your purified protein. Pierce Centrifuge Columns can be used in either spin or gravity-flow format. The spin format reduces sample processing time and makes column washing and elution more efficient. For gravity-flow procedures and when slow processing time is desired for binding steps with dilute samples, Column Extenders can be used to increase the column reservoir capacity. Use the any combination of processing techniques to get the best possible chromatography results.

Applications:

• Affinity purification, affinity chromatography
• Immunodepletion
• Ion exchange resins
• Gel filtration, desalting, buffer exchange

Related Products

• Pierce™ Centrifuge Columns, 2 mL (89896)
• Pierce™ Centrifuge Columns, 5 mL (89897)
• Pierce™ Centrifuge Columns, 10 mL (89898)
• Column Extenders for Pierce™ Centrifuge Columns (69707)

Specifications

• Capacity (Metric): 0.8 mL
• Product Line: Pierce™
• Type: Spin Column
• Column Type: Empty Column, Polyethylene Filter

• Content And Storage: Store at room temperature.
• Quantity: 4 x 50 Columns
• Format: Microcentrifuge, Spin Column

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.