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Thermo Scientific™ Phusion™ Plus PCR Master Mix

Thermo Scientific Phusion Plus PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs.

Supplier:  Thermo Scientific™ F631L

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Catalog No. F631L

  • $815.65 / Each of 1

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Description

Description

Thermo Scientific Phusion Plus PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs. The master mix format simplifies reaction setup with minimal pipetting steps and retains all features of the stand-alone format.

High fidelity—provides >100X greater sequence accuracy than Taq polymerase
Convenient setup—enables a universal annealing temperature of 60°C in PCR
Enhanced specificity—hot-start modification reduces non-specific amplification and primer degradation
Benchtop stability—automation enabled due to 24-hour stability of assembled reactions
High inhibitor tolerance—works with DNA of suboptimal purity

Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing. It is a Pyrococcus-like proofreading DNA polymerase fused to a processivity-enhancing domain, enabling Phusion Plus PCR Master Mix to generate PCR sequences with high accuracy, sensitivity, and inhibitor tolerance. Its novel reaction buffer includes proprietary additives that stabilize primer/template duplexes during the annealing step at 60°C. This unique buffer formulation increases PCR specificity without the need to optimize annealing temperature for each primer pair, helping save time and avoid mistakes in PCR runs. Furthermore, with Phusion Plus PCR Master Mix and its universal annealing feature, different PCR reactions can be cycled together using the same protocol.

Phusion Plus PCR Master Mix is inactive at ambient temperatures due to its hot-start mechanism mediated by Affibody molecules, allowing setup and storage of assembled PCR reactions at room temperature. Enzyme activity is fully restored after the initial denaturation step.

Applications
• High-fidelity PCR
• Cloning
• Template generation for sequencing
• GC-rich PCR
• Long-range PCR (up to 20 kb)
• Mutagenesis
• High-throughput PCR

TRUSTED_SUSTAINABILITY
Specifications

Specifications

• 2X Phusion Plus PCR Master Mix (10 x 1.25 mL)
• 5X Phusion GC Enhancer (4 x 1.25 mL)
• Nuclease-free water (10 x 1.25 mL)

Store at –15°C to –25°C
Phusion Plus DNA Polymerase
Dry Ice
2X
Built-In Hot Start
Blunt
20 kb or less
High
15 to 30 sec./kb
Hot-start PCR, High-fidelity PCR
>100X
500 Reactions
Ready to use master mix
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