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Thermo Scientific™ Phusion Hot Start II High-Fidelity PCR Master Mix

The most accurate hot start DNA polymerase on the market featuring extreme specificity and improved performance.

$283.00 - $880.65

Specifications

Content And Storage Includes:

• 2X Phusion Hot Start II High-Fidelity PCR Master Mix (provides 1.5 mM MgCl2 in the final 1X concentration)
• Water, nuclease-free
• DMSO

Store at -20°C.
Format Tube
GC-Rich PCR Performance Medium
Polymerase Phusion Hot Start II High-Fidelity DNA Polymerase
Reaction Speed Fast
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Products 2
Catalog Number Mfr. No. No. of Reactions Price Quantity & Availability  
Catalog Number Mfr. No. No. of Reactions Price Quantity & Availability  
F565L
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Thermo Scientific™
F565L
500
Each for $880.65

Save 18%

Reg : $1,080.00

The following deviations and disclosures may produce prices below stated discounts but will not trigger price reductions: Price shown reflects a temporary, promotional price reduction. No other purchase or commercial obligation required to receive the reduced price. Price may change at any time. Promotional price valid on web orders only. Your contract pricing may differ. Other exclusions apply.
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Add to Cart
 
F565S
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Thermo Scientific™
F565S
100
Each for $283.00
Add to Cart
 
Description

Description

Phusion Hot Start II High Fidelity DNA Polymerase combines the Phusion DNA Polymerase and a reversibly bound, specific Affibody ligand. Affibody inhibits the DNA polymerase activity at room temperature and thus prevents the amplification of nonspecific products.

The Affibody ligand also inhibits the 3‡→5‡ exonuclease activity of the polymerase, preventing degradation of primers and template DNA during reaction set up. At polymerization temperatures, the ligand is released, rendering the polymerase fully active. Phusion Hot Start II DNA Polymerase does not require a separate activation step in the PCR protocol as it is immediately reactivated at high temperatures.

New Phusion Green Hot Start II High-Fidelity DNA Polymerase is a combination of Phusion Hot Start II DNA Polymerase and 5X Phusion Green Buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with excelent performance of Phusion Hot Start II DNA Polymerase and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Thermo Scientific Phusion Hot Start II High Fidelity DNA Polymerase is the most accurate hot start DNA polymerase on the market. It combines the Phusion DNA Polymerase and a reversibly bound, specific Affibody ligand. Affibody inhibits the DNA polymerase activity at room temperature and thus prevents the amplification of nonspecific products.
The Affibody ligand also inhibits the 3'→5' exonuclease activity of the polymerase, preventing degradation of primers and template DNA during reaction set up. At polymerization temperatures, the ligand is released, rendering the polymerase fully active. Phusion Hot Start II DNA Polymerase does not require a separate activation step in the PCR protocol as it is immediately reactivated at high temperatures.
New Phusion Green Hot Start II High-Fidelity DNA Polymerase is a combination of Phusion Hot Start II DNA Polymerase and 5X Phusion Green Buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with excelent performance of Phusion Hot Start II DNA Polymerase and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.


Highlights

  • Reaction set up can be done at room temperature
  • Reduces non-specific amplification
  • Prevents primer degradation during set up
  • Zero-time reactivation due to unique hot start technology
  • Extreme fidelity (52X Taq)
  • Speed of the polymerase allows short extension times
  • Robust reactions, minimal optimization needed
  • Increased product yields with minimal enzyme amounts
  • Direct loading on gel with Green Buffer
Applications
  • High-fidelity PCR
  • High throughput
  • Difficult (GC-rich) templates
  • Template generation for sequencing
  • Multiplex PCR
  • Long-range PCR
  • Cloning
  • Mutagenesis†
  • Microarray
†Phusion Site-Directed Mutagenesis Kit also available.

Includes
Phusion Hot Start II High-Fidelity DNA Polymerase
  • Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/μL)
  • 5X Phusion HF Buffer
  • 5X Phusion GC Buffer
  • DMSO
  • 50 mM MgCl2 solution
Phusion Green Hot Start II High-Fidelity DNA Polymerase
  • Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/μL)
  • 5X Phusion Green HF Buffer
  • 5X Phusion Green GC Buffer
  • DMSO
  • 50 mM MgCl2 solution
Both Phusion and Phusion Green Buffers provide 1.5 mM MgCl2 in the final 1X concentration.

 

Hazardous: No
Shelf Life: 24 Months
Shipping Condition: Dry Ice
Storage buffer: 20 mM Tris-HCl (pH 7.4 at 25°C), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, stabilizers, 200 μg/mL BSA, and 50% glycerol.
Storage Condition: −20°C

Specifications

Specifications

Includes:

• 2X Phusion Hot Start II High-Fidelity PCR Master Mix (provides 1.5 mM MgCl2 in the final 1X concentration)
• Water, nuclease-free
• DMSO

Store at -20°C.
Medium
Fast
Hot Start High-Fidelity PCR Master Mix
2X
Built-In Hot Start
SuperMix or Master Mix
DNA
Tube
Phusion Hot Start II High-Fidelity DNA Polymerase
Phusion
Cloning
52 X
Blunt
20 kb or Less
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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