Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerase & dNTP Mix (10 mM each)

This package includes Phusion High-Fidelity DNA Polymerase (4 x 500 U) and high quality dNTP Mix (10 mM each, 2 x 1 mL).

Supplier:  Thermo Scientific™ F530N

Catalog No. F530N

Description

Phusion™ High-Fidelity DNA Polymerase

Thermo Scientific™ Phusion™ High-Fidelity DNA polymerases set a high standard for high performance PCR. Featuring an error rate 52-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion™ High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion™ DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.

• High fidelity (52X Taq)
• Fast PCR due to short extension times (15-30 s/kb)
• Robust performance, minimal optimization needed
• High yields of PCR products with minimal enzyme amounts

Using Phusion™ DNA polymerases

Annealing rules for Phusion™ DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).

dNTP Mix

Thermo Scientific™ dNTP Mix contains pre-mixed aqueous solutions of dATP, dCTP, dGTP, and dTTP, each at a final concentration of 10 mM. The nucleotides have greater than 99% purity and are free of nuclease activities, as well as human and E. coli DNA. Mixes offer the possibility of reducing the number of pipetting steps and the risk of reaction setup errors.

• Greater than 99% purity confirmed by HPLC
• Free of human and E. coli DNA
• Stable after multiple freeze-thaw cycles
• Up to 95% of dNTPs remain in triphosphate form after 7 weeks at room temperature
• Up to 90% of dNTPs remain in triphosphate form after 30 cycles of PCR (1 minute at 94°C; 3 minutes at 72°C)

For use in all molecular biology applications, including PCR, real-time PCR, high fidelity and long PCR, LAMP-PCR, cDNA synthesis, RT-PCR, RDA, MDA, DNA labeling, and DNA sequencing.

Specifications

• Content And Storage: • Phusion DNA Polymerase (2 U/µL)
  • 5X Phusion HF Buffer
  • 5X Phusion GC Buffer
  • DMSO
  • 50 mM MgCl₂ solution
  • 10 mM dNTP Mix
  
  Store at -5 to -30°C.
  
  
• Format: Kit
• PCR Method: 1-step RT-qPCR
• Reaction Speed: Fast
• Product Type: High-Fidelity DNA Polymerase
• Shipping Condition: Dry Ice
• For Use With (Application): Standard PCR, Cloning
• Fidelity (vs. Taq): 52 X
• No. of Reactions: 2000 Reactions
• Reaction Format: Separate Components
• Starting Material: DNA

• Detection Method: Primer-Probe
• GC-Rich PCR Performance: High
• Polymerase: Phusion High Fidelity DNA Polymerase
• Product Line: Phusion
• Purity or Quality Grade: HPLC
• Sufficient For: 2000 Reactions
• Concentration: 10 mM each
• Hot Start: No
• Overhang: Blunt
• Size (Final Product): 20 kb or Less
• Volume: 2 U/μL