Thermo Scientific™ Phusion™ HF and GC Buffer Packs

5X Phusion Green buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel.

Catalog Number	Mfr. No.	Product Type	Color
F518L	Thermo Scientific™ F518L	HF Buffer Pack	Colorless
F519L	Thermo Scientific™ F519L	GC Buffer Pack	Colorless
F520L	Thermo Scientific™ F520L	Detergent-free FF Buffer Pack	Colorless
F521L	Thermo Scientific™ F521L	Detergent-free GC Buffer Pack	Colorless
F538L	Thermo Scientific™ F538L	HF Buffer Pack	Green
F539L	Thermo Scientific™ F539L	GC Buffer Pack	Green

Description

The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10^-7) is lower than that in GC Buffer (9.5 x 10^-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.

5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases:

• 5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free))
• 5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free))

Specifications

• Polymerase: Phusion High-Fidelity DNA Polymerase
• For Use With (Application): Standard PCR, High-fidelity PCR
• Volume: 4 x 1.5 mL

• Shipping Condition: Wet or Dry Ice
• Starting Material: DNA

For Research Use Only. Not for use in diagnostic procedures.