Thermo Scientific™ Phire Green Hot Start II DNA Polymerase

A novel hot start DNA Polymerase for routine and high throughput PCR applications requiring speed and specifity.

Catalog Number	Mfr. No.	Sufficient For
F124L	Thermo Scientific™ F124L	1000 x 50 μL Reactions
F124S	Thermo Scientific™ F124S	200 x 50 μL Reactions

Description

Phire Hot Start II DNA Polymerase is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields. These features are achieved through advanced protein engineering of the polymerase. It incorporates a unique double-stranded DNA binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to Taq DNA polymerase.

The hot start modification of Phire Hot Start II DNA Polymerase is based on the same Affibody inactivation technology utilized by Phusion Hot Start II DNA Polymerase. This technology increases the specificity of the PCR reaction with no additional time required for initial activation of the enzyme.

New Phire Green format is a combination of Phire Hot Start II DNA Polymerase and 5X Phire Green Buffer. The buffer includes a density reagent and tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Thermo Scientific Phire Hot Start II DNA Polymerase is a novel PCR enzyme for routine and high throughput PCR applications. It outperforms every Taq-based hot start polymerase on the market. Phire Hot Start II DNA Polymerase is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields. These features are achieved through advanced protein engineering of the polymerase. It incorporates a unique double-stranded DNA binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to Taq DNA polymerase.
The hot start modification of Phire Hot Start II DNA Polymerase is based on the same Affibody inactivation technology utilized by Phusion Hot Start II DNA Polymerase. This technology increases the specificity of the PCR reaction with no additional time required for initial activation of the enzyme.
New Phire Green format is a combination of Phire Hot Start II DNA Polymerase and 5X Phire Green Buffer. The buffer includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.


Highlights

• Quick hot start - No reactivation step
• Fast enzyme - Amplify four times faster than with hot start Taq
• Robust - Minimal reaction optimization due to high inhibitor tolerance
• High yields - Abundant products due to high efficiency
• Longer PCR products - Amplify significantly longer DNA fragments than with any hot start Taq
• Direct loading on gel with Green Buffer

Applications

• Hot Start PCR
• Routine PCR
• Non-high fidelity PCR
• Fast PCR
• High throughput PCR
• Genotyping

Includes
Phire Hot Start II DNA Polymerase

• Phire Hot Start II DNA Polymerase
• 5X Phire Reaction Buffer (provides 1.5 mM MgCl₂ in the final 1X concentration)
• DMSO

Phire Green Hot Start II DNA Polymerase

• Phire Hot Start II DNA Polymerase
• 5X Phire Green Reaction Buffer (provides 1.5 mM MgCl₂ in the final 1X concentration)
• DMSO

Specifications

• GC-Rich PCR Performance: Medium
• Reaction Speed: Fast
• Product Type: Hot Start High-Fidelity DNA Polymerase
• Concentration: 50X
• Hot Start: Built-In Hot Start
• Reaction Format: Standalone

• Polymerase: Phire Hot Start II DNA polymerase
• Product Line: Phire
• For Use With (Application): Cloning
• Fidelity (vs. Taq): 2 X
• Overhang: Blunt
• Starting Material: DNA