Novus Biologicals™ Pam3CSK4, TLR1 and TLR2 Ligand

Applications: Functional, In vitro assay, In vivo assay, Ligand Activation

Supplier:  Novus Biologicals™ NBP2252970.025MG

Catalog No. NBP22529725

Description

Pam3CSK4, TLR1 and TLR2 Ligand is a synthetic tripalmitoylated lipohexapeptide analog of the immunologically active N-terminal portion of bacterial lipoprotein. It activates monocytes and macrophages and is also a potent activator of proinflammatory transcription factor NF-kB. Pam3CSK4, TLR1 and TLR2 Ligand is recognized by a heterodimer formed between TLR1 and TLR2. Pam3CSK4, TLR1 and TLR2 Ligand stimulation: If your cell line does not naturally express TLR1+ TLR2, co-transfect with plasmids pCMV/TLR1and pCMV/TLR2. Forty-eight hours after transfection, stimulate cells with 10 to 100ng/ml of Pam3CSK4 for 6 to 24 hours. Determine Pam3CSK4 stimulation using appropriate detection assays. Buffer: Sterile water

Specifications

• Concentration: 0.5 mg/mL
• Quantity: 0.025 mg
• Target: Pam3CSK4, TLR1 and TLR2 Ligand

• Content And Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• For Use With (Application): Activation of TLR1 and TLR2 heterodimer. Stimulation of TLR1 and TLR2 has been reported with 10 to 100ng/mL. Use in vitro assay reported in scientific literature (PMID: 24105263) Use in functional reported in scientific literature (PMID 25511699). Use in In-vivo reported in scientific literature (PMID 25466255). Use in ligand activation reported in multiple pieces scientific literature.
• Species: Human/Mouse