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Thermo Scientific™ Mem-PER™ Plus Membrane Protein Extraction Kit
Perform small-scale solubilization and enrichment of integral membrane proteins and membrane-associated proteins in a simple reagent-based procedure.
Supplier: Thermo Scientific™ 89842
Description
Perform small-scale solubilization and enrichment of integral membrane proteins and membrane-associated proteins in a simple reagent-based procedure using Thermo Scientific™ Mem-PER Plus Membrane Protein Extraction Kit.
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Highlights:
Extraction and isolation – produces minimal cross-contamination (typically less than 10%) of cytosolic protein into the membrane protein fraction
Cells or tissues – effective for extraction from cultured mammalian cells and mammalian tissues
Downstream compatibility – analyze membrane protein extracts by SDS-PAGE, Western blotting, immunoprecipitation and protein assays
Fast and simple – isolation of membrane proteins in approximately one hour
No special equipment required – only a benchtop microcentrifuge, tubes, homogenizers, and pipettors are needed
Includes:
Kit contains a cell wash solution, permeabilization buffer, and solubilization buffer.
Specifications
Protein Extraction Kit | |
Liquid | |
Cell Lysis Buffer, Detergent Solution, Subcellular Fractionation | |
1 Kit |
Upon receipt store Cell Wash Solution and Solubilization Buffer at 4°C. Aliquot and store Permeabilization Buffer at -20°C to avoid freeze/thaws. Product is shipped on ice. | |
Mem-PER™ | |
50 Samples |
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For Research Use Only. Not for use in diagnostic procedures.