Thermo Scientific™ tRNA for LightShift™ Chemiluminescent RNA EMSA Kit
Study RNA-protein interactions with this non-radioactive EMSA kit for gel shift assays.
Supplier: Thermo Scientific™ 20159
Thermo Scientific™ LightShift Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).The RNA EMSA Kit uses biotinylated RNA probes and a chemiluminescent substrate system to achieve fast and safe detection of RNA-protein complexes with sensitivity equivalent to traditional 32P-isotopic methods. The complete kit comes with all reagents required to set up and optimize protein-RNA binding conditions, a positive control for protein-RNA interactions and reagents for chemiluminescent detection of the nucleic acid interaction.
Sensitive – chemiluminescent detection comparable to radioactive detection
Time-saving – develop X-ray films after 1- to 5-minute exposures, versus 16 hour exposures needed with radioactive systems
Flexible – compatible with RNA probes biotinylated by different methods
Easy to use – complete kit includes optimized reagents for binding reactions and RNA probe detection
Non-radioactive – eliminate waste concerns from radioactive RNA probes
|Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, but do not store above -70°C.|
|tRNA for LightShift RNA EMSA Kit|
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