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Invitrogen™ Platinum™ Taq DNA Polymerase
Convenient and reliable “hot start” thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase
Supplier: Invitrogen™ 10966034
Includes: Each box contains: Platinum Taq DNA Polymerase; 10X PCR Buffer (no magnesium chloride); 50mM magnesium chloride; KB Extender
Description
Use Platinum Taq DNA Polymerase for the amplification of DNA from complex genomic, viral, and plasmid templates, as well as in RT-PCR. The “hot start” property of the enzyme preparation is conferred by thermolabile monoclonal antibodies that render Taq DNA polymerase inactive until the initial PCR denaturation step.
- Convenience: assemble PCR reactions at room temperature
- Specificity: “hot start” kinetics reduce nonspecific primer annealing, improving product yield
- Product size: use for PCR products up to 10kb
- Flexibility: optional KB Extender gives more versatility in PCR assays
Using PlatinumTaq DNA Polymerase
- Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a nontemplate-dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends, and has a 5'→3' exonuclease activity (but not 3'→5' exonuclease activity)
- PCR products generated with PlatinumTaq DNA Polymerase may be used in the same downstream applications without protocol modifications
Amplification of Bisulfite-Treated DNA, Chromatin Biology, Chromatin Immunoprecipitation (ChIP), Fast PCR, Hot Start PCR, Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Noncoding RNA Research, Routine PCR
Order Info
Shipping Condition: Approved for shipment on wet or dry ice
Specifications
• 1 x 120 μL Platinum Taq DNA Polymerase • 3 x 1.25 mL 10X PCR Buffer (no magnesium chloride) • 1 x 1 mL 50 mM MgCl2 • 1 x 1.3 mL KB Extender Store at -10 to -30°C in a non-frost-free freezer. |
|
Tube | |
Platinum Taq DNA Polymerase | |
5' - 3' | |
DNA Polymerase | |
Approved for shipment on Wet or Dry Ice | |
1 X | |
600 Reactions | |
Separate Components | |
DNA |
Primer-probe | |
Low | |
Fast or Standard | |
Platinum™ | |
600 Reactions | |
600 Reactions | |
Built-In Hot Start | |
3'-A | |
5 kb or less |
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For Research Use Only. Not for use in diagnostic procedures.