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Invitrogen™ Plasmid pBR322

Name: Invitrogen&trade; Plasmid pBR322
Brand: Invitrogen&trade;
SKU: 15-367-014
Price: 348.00 USD

Prepacked column

Supplier: Invitrogen™ 15367014

Catalog No. 15-367-014

$348.00 / Each of 1

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Includes: pBR322 is supplied at 0.25μg/μL in 10mM Tris-HCl (pH 7.4), 5mM NaCl, 0.1mM EDTA.

Description

Plasmid pBR322 (1) contains three unique restriction endonuclease recognition sites within the !-lactamase (AmpR) gene and eight unique sites within the TetR gene. The plasmid, purified from DH10B E. coli, also has 14 non-selectable unique restriction endonuclease recognition sites. Unique cleavage sites for Hind III and Cla I are found in the promoter of the tetracycline resistance gene. Insertion of DNA at either of these two sites usually results in loss of tetracycline resistance. Performance and Quality Testing: Purity, DNA structure, and selected restriction endonuclease fragmentation patterns are verified by agarose gel analysis. Transformation of E. coli HB101 to confirm AmpR and TetR is confirmed by plating on selective medium.

Cloning, Restriction Enzyme Cloning

Order Info

Approved for shipment on Wet or Dry ice

Specifications

Cloning Method	Restriction Enzyme/ MCS
Content And Storage	pBR322 is supplied at 0.25 μg/μl in 10 mM Tris-HCl (pH 7.4), 5 mM NaCl, 0.1 mM EDTA. Store at +4°C.
Product Type	Plasmid
Shipping Condition	Approved for shipment on Wet or Dry Ice

Concentration	0.25 μg/μl
For Use With (Application)	Cloning
Quantity	25 μg
Vector	pBR322

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