Test
Optimize cloning with Thermo Scientific™ pBR322 DNA, a 4361 bp double-stranded closed circular medium copy plasmid DNA.
For expression of hROR gamma protein in mammalian cells, induction of IL-17A promoter activity
Prepacked column
For expression of hRUNX1 protein in mammalian cells, induction of FOXP3 promoter activity
Mammalian expression vector construct, pCMV/hRUNX1, containing coding sequence of human Runt-related transcription factor 1
Yeast Rapid Pure Miniprep Plasmid Isolation Kit designed for yeast cells. Can isolate plasmid from bacterial transformants for use in any downstream application (PCR, sequencing, restriction enzyme digestion, labeling, etc).
pOET1C_6xHis Transfer Plasmid for high-yield protein production in insect cells.
For expression of hROR gamma (t) [del. 5-8] protein, which is a novel hROR gamma (t) isoform
For expression of hROR alpha protein in mammalian cells, induction of G6Pase promoter activity
pOET1 Transfer Plasmid for high-yield protein production in insect cells.
pOET6 BacMam Transfer Plasmid for high-yield protein production in mammalian cells.
NC2102003 PBKV 34-2 PLASMID IN E. COLI
Plasmid DNA from E. coli RRI pUC18, buffered aqueous solution | 9007-49-2 | MFCD00166896 | 10UG
The Zyppy-96 Plasmid Miniprep allows efficient isolation of plasmid DNA from E. coli, featuring a modified alkaline lysis system that is pellet-free. This completely bypasses tedious centrifugation, pelleting, and re-suspension steps common to conventional procedures. Instead, the uniquely formulated Deep Blue Lysis Buffer is added directly to the bacterial cultures in a 96-well block. Buffer neutralization and lysate separation steps are assisted with a specially designed Neutralization/Clearing Buffer. Eluted plasmid DNA is high quality, endotoxin-free, and well-suited for use in restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications including transfection.
The Monarch Plasmid Miniprep Kit is a rapid and reliable method for the purification of high quality plasmid DNA. This method employs standard cell resuspension, alkaline lysis, and neutralization steps, with the additional benefit of color indicators at certain steps to easily monitor completion. Unique wash buffers ensure salts, proteins, RNA and other cellular components are removed, allowing low-volume elution of concentrated, highly pure DNA. Protocols are fast and user friendly, saving you valuable time. Elution in as little as 30 ul provides concentrated DNA for use in downstream applications, such as restriction digests, DNA sequencing, PCR and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging.