Invitrogen™ AccuPrime™ Pfx SuperMix
High-fidelity, high-specificity amplification of DNA fragments
Supplier: Invitrogen™ 12344040
Includes: AccuPrime Pfx SuperMix is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 200 amplification reactions of 25μL each (4 x 1.125mL) are provided. AccuPrime Pfx SuperMix Components: 22U/mL Thermococcus species KOD thermostable polymerase complexed with anti-KOD antibodies, 66mM Tris-SO4 (pH 8.4), 30.8mM (NH4)2SO4, 11mM KCl, 1.1mM MgSO4, 330μM dNTPs, AccuPrime proteins, stabilizers.
Requires : Template and primers
AccuPrime Pfx SuperMix is a ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs for efficient PCR amplification. All you have to do is add template and primers, reducing set-up time by half.
- Achieve >26-times higher fidelity than with Taq DNA polymerase
- Amplify fragments up to 12kb
- Mminimize PCR optimization
- Produce products with blunt ends
- Built-in hot start
Using AccuPrime Pfx SuperMix
AccuPrime Pfx SuperMix is ideal for high-fidelity, high-specificity amplification of DNA fragments for applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading activity. Thermostable AccuPrime proteins enhance primer-template hybridization during every cycle, increasing specificity and yield.
Amplification of Bisulfite-Treated DNA, High Fidelity PCR, Hot Start PCR, Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research
Approved for shipment on Wet or Dry Ice
|• 4 × 1.125 mL AccuPrime Pfx SuperMix
Store at -20°C in a non-frost-free freezer.
|AccuPrime Pfx DNA Polymerase|
|3' - 5'|
|Approved for shipment on Wet or Dry Ice|
|SuperMix or Master Mix|
|Built-In Hot Start|
|15 kb or less|
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For Research Use Only. Not for use in diagnostic procedures.