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Invitrogen™ AccuPrime™ Pfx SuperMix
AccuPrime™ Pfx SuperMix is a ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs for efficient PCR amplification.
Supplier: Invitrogen™ 12344040
Includes: AccuPrime Pfx SuperMix is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 200 amplification reactions of 25μL each (4 x 1.125mL) are provided. AccuPrime Pfx SuperMix Components: 22U/mL Thermococcus species KOD thermostable polymerase complexed with anti-KOD antibodies, 66mM Tris-SO4 (pH 8.4), 30.8mM (NH4)2SO4, 11mM KCl, 1.1mM MgSO4, 330μM dNTPs, AccuPrime proteins, stabilizers.
Requires : Template and primers
Description
AccuPrime™ Pfx SuperMix is a ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs for efficient PCR amplification. All you have to do is add template and primers, reducing set-up time by half.
With AccuPrime™ Pfx SuperMix you will:
• Achieve >26-times higher fidelity than with Taq DNA polymerase
• Amplify fragments up to 12 kb
• Minimize PCR optimization
• Produce products with blunt ends
Using AccuPrime™ Pfx SuperMix
AccuPrime™ Pfx SuperMix is ideal for high-fidelity, high-specificity amplification of DNA fragments for applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading activity. Thermostable AccuPrime™ proteins enhance primer-template hybridization during every cycle, increasing specificity and yield.
Order Info
Approved for shipment on Wet or Dry Ice
Specifications
AccuPrime Pfx SuperMix (4 × 1.125 mL) Store at –20°C in a non-frost-free freezer. |
|
Low | |
Standard | |
Hot-start PCR, High-fidelity PCR | |
26X | |
200 Reactions | |
SuperMix or Master Mix |
Primer-probe | |
AccuPrime Pfx DNA Polymerase | |
Wet or Dry Ice | |
1.1X | |
Built-In Hot Start | |
Blunt | |
15 kb or less |
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For Research Use Only. Not for use in diagnostic procedures.