Cytiva HiScreen™ Sepharose™ Prepacked Fast Flow HIC Columns

Designed for method optimization and parameter screening

Supplier:  Cytiva 28926989

Catalog No. 45-002-538

Description

• Ready-to-use columns for convenient process development optimization
• Standard aliphatic/aromatic Hydrophobic Interaction Chromatography media for capture and intermediate purification
• Bed height of 10cm designed to allow method optimization and parameter screening
• Easily connected in series to achieve 20cm bed height
• Small bed volume of 4.7mL for fast result and minimal sample/buffer consumption
• Reproducible results, scalable to BioProcess columns packed with same chromatography media using same linear fluid velocity

• Bed dimension: 7.7 × 100mm
• Matrix: 4% cross-linked agarose/6% cross-linked agarose
• pH stability working range (media): 3 to 13
• Average particle size: 90μm
• Material (column hardware and column tube): polypropylene
• Column I.D.: 7.7mm
• Pressure max.: 1.5 bar (22psi)
• Flow velocity: <240/450cm/hr.

Specifications

• Buffer: 1M NaOH, 3M Ammonium Sulphate, 30% Isopropanol, 70% Ethanol, 10% Ethylene Glycol, 0.5% SDS, 6M Guanidine Hydrochloride, 8M Urea
• Bed Volume: 4.7mL
• Diameter (Metric) Inner: 7.7mm
• Height (English): 3.93 in.
• Flow Rate: <450cm/hr.
• Ligand Type: Phenyl
• pH Range: 3 to 13
• Support Type: 6% Cross-linked Agarose
• Column Type: Ion Exchange Column

• Product Type: HiScreen Phenyl (low sub) Sepharose 6 FF column
• Diameter (English) Inner: 0.3 in.
• Quantity: 1 x 4.7mL
• Height (Metric): 100mm
• Material: Polypropylene
• Max. Pressure: 1.5 bar
• Particle Size: 45 to 165μm
• Content And Storage: 4° to 30°C
• For Use With (Application): Convenient process development optimization parameter screening