Cytiva™ HiScreen™ Column with MabSelect™ VL Protein L Resin

These HiScreen™ columns contain Protein L resin for affinity capture of antibody Fabs, bispecifics, and other variants containing a kappa light chain.

Supplier:  Cytiva™ 17542015

Catalog No. 01-257-013

Description

As the diversity of antibody variants increases, so does the need for chromatography resins that can capture and purify these variants. As an alternative to protein A, protein L resin can be used to capture antibody Fabs, bispecific antibodies, and other molecules containing kappa variants of the variable light (VL) chain. Protein L binds human kappa light chain subtypes 1, 3, and 4 and mouse kappa light chain subtype 1.

Redesigned protein L ligand MabSelect™ VL protein L resin showed a two-fold increase in binding capacity compared to Cytiva™ Capto™ L chromatography resin. This higher capacity provides productivity advantages in research and biomanufacturing.

• A redesigned protein L ligand captures antibody Fabs, bispecifics, and other variants containing a kappa light chain with high binding capacity and improved alkaline stability
• High dynamic binding capacity for efficient capture of target molecules
• Stable when cleaned with 0.1 M NaOH, reducing risk for bioburden incidents
• Provides good resolution for product-related impurities in the capture of bispecific antibodies
• Convenient for method optimization and parameter screening enabled by the 10 cm bed height for efficient chromatography process development

Specifications

• Binding Capacity: 70 mg Human IgG/mL Resin at 6 min. Residence Time, 60 mg Human IgG/mL Resin at 4 min. Residence Time
• Diameter (English) Inner: 0.3 in.
• Flow Rate: <300 cm/hr.
• Particle Size: 60 μm
• Quantity: 1 Column
• Target: Antibody
• Format: Chromatography Column
• Column Type: Affinity

• Capacity (Metric): 4.7 mL
• Diameter (Metric) Inner: 7.7 mm
• Max. Operating Pressure: 3 bar
• Product Line: HiScreen™ MabSelect™
• For Use With (Application): Antibody Affinity Chromatography
• Type: Pre-packed Column
• Bed Height: 100 mm