Cytiva HiLoad™ 16/600 Superdex™ 200 pg

Use for separating MAbs from dimers and contaminants. Cytiva HiLoad™ 16/600 Superdex™ 200 pg is ideal for high-resolution preparative gel filtration chromatography.

Supplier:  Cytiva 28989335

Catalog No. 45-002-490

Includes:

• Dismantling tool
• Support screen
• Net ring (10μm)
• O-ring
• Stop plugs
• Instructions

Description

Superdex prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose. Dextran component determined the separation properties of these media. Steep selectivity curves give gppd resolving power for peptides and proteins in the molecular weight range Mr 10 000 600 000 (Superdex 200 pg).

• Used for separating MAbs from dimers and contaminants
• Short run times with high recovery
• Medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions
• Separations can be scaled up to production levels
• 0.06 in. fittings to connect to AKTA design systems
• Theoretical plates: >13000m-¹
• Flow rate: <1.6mL/min.
• Pressure max. (over the packed bed during operation): 3 bar [0.3MPa] (42psi)

• Media
• Average particle size: 34μm
• Matrix: composite of cross-linked agarose and dextran
• pH Stability working range: 3 to 12
• pH Stability cleaning range: 2 to 14
• Fractionation [Mr] globular proteins: 1 × 10^4-6 × 10⁵
• Fractionation [Mr] dextrans: 1 × 10^3-1 × 10⁵
• Shelf life from manufacture date: 5 years
• Chemical stability: all commonly used buffers, 1M NaOH, 0.1M HCl, 1M acetic acid, 8M urea, 6M guanidine HCl, 1% SDS, 70% ethanol, 30% iso-propanol, 30% acetonitrile

• Column
• Column I.D.: 0.62 in. (16mm)
• Inlet I.D.: 0.031 in. (0.8mm)
• Outlet I.D.: 0.031 in. (0.8mm)
• Chemical stability: resistant to aqueous solutions and solvents used in liquid chromatography. Not resistant to Acetone, Ketones, Chlorinated Hydrocarbons, Aliphatic Esters and Phenol
• Column hardware made from borosilicate glass, polyamide, fuororubber, Ethylene Propylene Diene (EPDM), ETFE, Superpolyoxymethylene
• Column tube made from borosilicate glass
• Bed support made from Polypropylene (PP) (reinforced glass fiber), Polyamide (PA)
• Porosity: 10μm

Should be avoided: >10% NaOH, >10% HCl, >5% acetic acid, and strong mineral acids

Order Info

HiLoad columns are prepacked XK columns; Bed volume: 120mL; Bed dimensions: 0.62 x 23.62 in. (16 x 600mm); Sample volume: 5mL

Specifications

• Sample Volume: <5 mL
• Content And Storage: 4°C to 30°C, 20% Ethanol