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Gibco™ ExpiSf™ CD Medium

Gibco™ ExpiSf™ CD Medium is chemically defined, yeastolate-free, serum-free, protein-free, animal origin-free medium specifically designed for high-density growth, transfection, and baculovirus infection of suspension-adapted Sf9 (Spodoptera frugiperda) insect cells.

Supplier:  Gibco™ A3767805

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Catalog No. A3767805


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Description

Description

  • Delivers high reproducibility and lot-to-lot reliability
  • Facilitates high-level expression of baculovirus-infected cultures, achieving protein yields three times higher than traditional insect expression platforms
  • Supports growth of suspension Sf9 cultures to densities of approximately 2 x 107 cells/mL, with >90% cell viability
  • Supports high-density infection of suspension Sf9 cells with recombinant baculovirus
  • Supports growth, transfection, and baculovirus infection of Sf9 cells in culture formats from 24-deep well plates to >3L in shaker flasks
  • Is a ready-to-use formulation, with no supplementation required
  • manufactured using an ISO 13485-certified process according to 21 CFR 820 medical device current good manufacturing practices (cGMP)

ExpiSf™ CD Medium is a core component of the ExpiSf Expression System. For highest level protein expression, the medium is used in combination with a matched protein expression enhancer, ExpiSf Enhancer, which is supplied as part of the ExpiSf Protein Production Kit (Cat. No. A3767806). Sf21 and High Five™ cells adapted to other commercially-available serum-free media may be adapted for growth in ExpiSf CD Medium; although, sequential adaptation is required and high-density growth isn’t guaranteed. ExpiSf™ CD Medium is not recommended for adherent cell cultures. Cells are typically grown in Erlenmeyer-style flasks on a shaker platform in a non-humidified, non-CO2 cell culture incubator.

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Specifications

Specifications

20 L
Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free
Liquid
ExpiSf™
20 L bag, store in the dark at 2–8°C

Shelf Life: 12 months from date of manufacture

Insect Cells
Room Temperature
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WARNING: Reproductive Harm - www.P65Warnings.ca.gov
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