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Gibco™ ExpiSf™ CD Medium
Gibco™ ExpiSf™ CD Medium is chemically defined, yeastolate-free, serum-free, protein-free, animal origin-free medium specifically designed for high-density growth, transfection, and baculovirus infection of suspension-adapted Sf9 (Spodoptera frugiperda) insect cells.
Supplier: Gibco™ A3767803
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Description
- Delivers high reproducibility and lot-to-lot reliability
- Facilitates high-level expression of baculovirus-infected cultures, achieving protein yields three times higher than traditional insect expression platforms
- Supports growth of suspension Sf9 cultures to densities of approximately 2 x 107 cells/mL, with >90% cell viability
- Supports high-density infection of suspension Sf9 cells with recombinant baculovirus
- Supports growth, transfection, and baculovirus infection of Sf9 cells in culture formats from 24-deep well plates to >3L in shaker flasks
- Is a ready-to-use formulation, with no supplementation required
- manufactured using an ISO 13485-certified process according to 21 CFR 820 medical device current good manufacturing practices (cGMP)
ExpiSf™ CD Medium is a core component of the ExpiSf Expression System. For highest level protein expression, the medium is used in combination with a matched protein expression enhancer, ExpiSf Enhancer, which is supplied as part of the ExpiSf Protein Production Kit (Cat. No. A3767806). Sf21 and High Five™ cells adapted to other commercially-available serum-free media may be adapted for growth in ExpiSf CD Medium; although, sequential adaptation is required and high-density growth isn’t guaranteed. ExpiSf™ CD Medium is not recommended for adherent cell cultures. Cells are typically grown in Erlenmeyer-style flasks on a shaker platform in a non-humidified, non-CO2 cell culture incubator.
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Specifications
6 x 1000 mL | |
Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free | |
Liquid | |
ExpiSf™ |
6 x 1000 mL bottle, store in the dark at 2–8°C Shelf Life: 12 months from date of manufacture |
|
Insect Cells | |
Room Temperature |
Safety and Handling
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