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Edvotek™ Affinity Chromatography of Glucose-Binding Proteins

Isolate a carbohydrate-binding lectin protein from an extract of jack bean meal.

Supplier:  Edvotek™ 277

Catalog No. S05653

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  • Instructions
  • Affinity gel
  • Jack bean meal
  • NaCl, Dextrose
  • Con A Control
  • Horseradish peroxidase
  • ABTS Substrate
  • Standard dilution buffer
  • Membrane
  • Columns (syringe) and column tips
  • Cheesecloth for column
  • 50 ml conical tubes
  • Transfer pipets
  • Petri dishes



Affinity chromatography is applied to the purification of biologically active molecules. A ligand is coupled to an insoluble support. The specific protein binds to the ligand, which results in the separation of a specific protein from a mixture.

  • Students prepare a seed extract, fractionate the extract by affinity chromatography and elute the bound glucose-binding protein
  • As a second experiment, the presence of biological activity is determined by an immunoblot enzyme assay
  • Requires two hours

All You Need

  • shaking platform or vortex
  • rotating or rocking platform
  • clinical centrifuge
  • ring stands with clamps for columns
  • microteset tubes or small glass test tubes for collecting fractions
  • test tubes (15 ml) to collect elutant
  • 50 ml
  • 100 ml beakers or flasks
  • beaker or flask
  • graduated cylinders: 10 ml
  • 100 ml
  • and 250 ml
  • 10 ml pipets
  • forceps
  • distilled water
  • filter paper.

Order Info

For 10 groups.

Recommended Storage

Some components require refrigerator storage



Affinity Chromatography Kit
For Purification of Biologically Active Molecules
Instructions, affinity gel, jack bean meal, various solutions and buffers, petri plates, columns with tips, conical tubes, membranes, and transfer pipets
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