Edvotek™ Affinity Chromatography of Glucose-Binding Proteins
Isolate a carbohydrate-binding lectin protein from an extract of jack bean meal.
Supplier: Edvotek™ 277
Affinity chromatography is applied to the purification of biologically active molecules. A ligand is coupled to an insoluble support. The specific protein binds to the ligand, which results in the separation of a specific protein from a mixture.
- Students prepare a seed extract, fractionate the extract by affinity chromatography and elute the bound glucose-binding protein
- As a second experiment, the presence of biological activity is determined by an immunoblot enzyme assay
- Requires two hours
All You Need
- shaking platform or vortex
- rotating or rocking platform
- clinical centrifuge
- ring stands with clamps for columns
- microteset tubes or small glass test tubes for collecting fractions
- test tubes (15 ml) to collect elutant
- 50 ml
- 100 ml beakers or flasks
- beaker or flask
- graduated cylinders: 10 ml
- 100 ml
- and 250 ml
- 10 ml pipets
- distilled water
- filter paper.
For 10 groups.
Some components require refrigerator storage
|Affinity Chromatography Kit
|For Purification of Biologically Active Molecules
|Instructions, affinity gel, jack bean meal, various solutions and buffers, petri plates, columns with tips, conical tubes, membranes, and transfer pipets
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