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Gibco™ ExpiCHO™ Expression Medium
A GMP-manufactured, chemically-defined, serum-free, protein-free, animal origin–free medium specifically designed for high-density growth and transfection of suspension-adapted Chinese hamster ovary (CHO) cells
Specifications
Content And Storage | Storage conditions: in the dark at 2-8°C Shipping conditions: ambient Shelf life: 12 months from date of manufacture |
---|---|
Cell Line | ExpiCHO-S™, CHO-S, SFM Adapted |
Classification | Animal Origin-free, Chemically-defined, Protein-free, Serum-free |
Culture Type | Suspension Cell Culture |
Form | Liquid |
Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
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Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
A2910001
|
Gibco™
A2910001 |
1000 mL |
Each for $479.00
|
N/A
|
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A2910002
|
Gibco™
A2910002 |
6 x 1 L |
Each for $2,585.00
|
N/A
|
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A2910004
|
Gibco™
A2910004 |
20 L |
Each for $7,880.00
|
N/A | |||||
A2910003
|
Gibco™
A2910003 |
10 L |
Each for $4,100.00
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N/A
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Description
ExpiCHO Expression Medium is specifically designed for high-density growth and transfection of suspension-adapted Chinese hamster ovary (CHO) cells. This medium is GMP-manufactured, chemically-defined, serum-free, protein-free, and animal origin–free. It is a core component of the ExpiCHO Expression System Kit (Cat. No. A29133) for scalable transient protein production. To achieve the highest levels of transient protein expression, the medium is matched to a culture feed, ExpiCHO Feed, also included in the system kit and transfection kits.With ExpiCHO Expression Medium you can:
• Grow suspension CHO cultures to densities of over 2 x 107 cells per mL, with >95% cell viability
• Achieve high transfection efficiencies of >90% when coupled with ExpiFectamine CHO transfection reagent
• Transiently transfect suspension CHO cells at a density of 6 x 106 cells per mL
• Achieve yields up to 3 g per L of recombinant protein of high-density transiently transfected cultures by sustained, high-level expression
• Grow and transfect CHO cells at volumes of <1 mL up to 5 L in culture formats from multiwell plates up to 5 L shake flask
ExpiCHO Expression Medium is ready to use. It is formulated with GlutaMAX-I reagent and does not require supplementation. It does not contain phenol red. The chemically-defined, animal origin free formulation results in high reproducibility and lot-to-lot reliability. ExpiCHO Expression Medium is not recommended for adherent cell cultures.
Specifications
Storage conditions: in the dark at 2-8°C Shipping conditions: ambient Shelf life: 12 months from date of manufacture |
|
Animal Origin-free, Chemically-defined, Protein-free, Serum-free | |
Liquid | |
Serum-free | |
No Phenol Red | |
Room Temperature |
ExpiCHO-S™, CHO-S, SFM Adapted | |
Suspension Cell Culture | |
CHO Expression Medium | |
GlutaMAX | |
ExpiCHO™ |
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