Gibco™ ExpiCHO™ Expression Medium

A GMP-manufactured, chemically-defined, serum-free, protein-free, animal origin–free medium specifically designed for high-density growth and transfection of suspension-adapted Chinese hamster ovary (CHO) cells

Supplier:  Gibco™ A2910003

Catalog No. A2910003

Description

ExpiCHO™ Expression Medium is a GMP-manufactured, chemically-defined, serum-free, protein-free, animal origin–free medium specifically designed for high-density growth and transfection of suspension-adapted Chinese hamster ovary (CHO) cells. It is a core component of the ExpiCHO™ Expression System Kit (Cat. No. A29133) for scalable transient protein production.

ExpiCHO Expression Medium:

Supports growth of suspension CHO cultures to densities of over 2 x 10⁷ cells per mL, with >95% cell viability

Uses a transfection-compatible, serum-free suspension culture medium that enables high transfection efficiencies of >90% with ExpiFectamine™ CHO transfection reagent

Supports transient transfection of suspension CHO cells at a density of 6 x 10⁶ cells per mL

Facilitates sustained, high-level expression of high-density transiently transfected cultures, achieving yields of up to 3 g per L of recombinant protein

Supports growth and transfection of CHO cells in culture formats from <1 mL in multi-well plates to >10 L in benchtop bioreactors

Does not contain phenol red

ExpiCHO Expression Medium is formulated with GlutaMAX™-I reagent. It is ready to use, with no supplementation required. The chemically-defined formulation results in high reproducibility and lot-to-lot reliability. For highest level transient protein expression, the medium is used in combination with a matched culture feed, ExpiCHO™ Feed, which is supplied as part of the ExpiFectamine™ CHO Transfection Kit (Cat. No. A29129). ExpiCHO Expression Medium is not recommended for adherent cell cultures. Cells are typically grown in Erlenmeyer-style flasks on a shaker platform in a humidified, CO₂ cell culture incubator. However, cultures are scalable from volumes of <1 mL in multi-well plates to >10 L in bioreactors.

Specifications

• Content And Storage: Storage conditions: in the dark at 2-8°C
  Shipping conditions: ambient
  Shelf life: 12 months from date of manufacture
• Classification: Animal Origin-free, Chemically-defined, Protein-free, Serum-free
• Form: Liquid
• Serum Level: Serum-free
• Without Additives: No Phenol Red
• Product Line: ExpiCHO™

• Cell Line: CHO-S, SFM Adapted
• Culture Type: Suspension Cell Culture
• Product Type: CHO Expression Medium
• With Additives: GlutaMAX
• Format: Universal Bag
• Shipping Condition: Room Temperature