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Promega Caspase-Glo™ 3/7 Assay Kit
Caspase-Glo™ 3/7 Assay is a homogeneous luminescent assay that measures caspase-3/7 activities
Supplier: Promega ZG8092
Includes: Caspase-Glo 3/7 Substrate; Caspase-Glo 3/7 Buffer
Description
Utilizes a proluminescent Caspase-3/7 DEVD-aminoluciferin substrate and a proprietary thermostable luciferase in a single reagent optimized for Caspase-3/7 activity, luciferase activity, and cell lysis. Stabilized luciferase improves assay performance across a wide range of assay conditions and makes the assay less likely to be affected by compound interference than other luminescent-, fluorescent-, or colorimetric-based assays.
- Designed for use with 96- or 384-well plates using either purified enzyme or cells in culture as sample
- Requires less enzyme or fewer cells than other caspase formats due to low background luminescence
- Requires no sample preparation, manipulation, or extended incubation times—maximum sensitivity is achieved in as little as one-half to one hour
- “Add-mix-measure” protocol makes the assay easy to automate
- Extended-glow signal allows the plates to be read over a three-hour period for batch processing—no injectors required
Specifications
10 x 100 mL | |
-20°C | |
Liquid |
7.4 | |
96- or 384-well plates using either purified enzyme or cells in culture as sample |
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