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Molecular Probes™ CaptAvidin™ Agarose (Sedimented Bead Suspension)
CaptAvidin™ Agarose (Sedimented Bead Suspension)
Supplier: Molecular Probes™ C21386
Description
CaptAvidin™ biotin-binding protein conjugated to agarose is designed for use in affinity chromatography. CaptAvidin™ biotin-binding protein contains a nitrated tyrosine in its biotin-binding site. This chemical modification permits dissociation of the avidin-biotin complex under milder conditions than the traditional avidin-biotin complex. At pH 4.0, CaptAvidin™ biotin-binding protein binds biotin tightly with a Ka of ∼109M-1. This association can then be reversed at pH 10, allowing complete dissociation of the avidin-biotin complex. This is in contrast to the harsh conditions (8 M guanidine hydrochloride, pH 1.5) needed to dissociate the avidin-biotin complex.
Specifications
| Store in refrigerator at 2°C to 8°C | |
| Wet Ice | |
| Agarose |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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