BD Sphero™ Rainbow Calibration Particles

For flow cytometry calibration and alignment

Supplier:  BD 556298

Catalog No. BDB556298

Description

• For flow cytometry calibration and alignment
• Have emission spectra compatible with many common fluorophores used for immunofluorescent staining with flow cytometric analysis
• Very small coefficients of variation in both size and fluorescence
• Contain water insoluble fluorescent dyes which are very stable, allowing them to be sterilized by washing with 70% alcohol
• Provided in convenient dropper bottles containing 5mL of particle suspension at a concentration of 10⁷ particles/mL
• Polystyrene

Rainbow Calibration Particles (6 Peaks)

• Each vial contains a mixture of Rainbow Particles that are dyed to six different fluorescent intensities

Rainbow Calibration Particles (8 Peaks)

• The vial contains a mixture of Rainbow Particles that are dyed to eight different fluorescent intensities

Rainbow Fluorescent Particles (High Intensity)

• Each vial contains a single population of Rainbow Particles that are dyed to a single fluorescent intensity
• The intensity of this particle corresponds to the brightest intesity of peak in the Rainbow Calibration Particles, 3.0-3.4μm (Mfr. No. 556286)

Rainbow Fluorescent Particles (Mid-range FL1 Fluorescence)

• Each vial contains a single population of Rainbow Particles that are dyed to a single fluorescent intensity

Blank Calibration Particles

• Each vial contains a single population of Blank Calibration Particles that are dyed to a single fluorescent intensity
• The intensity of this particle corresponds to the dimmest intensity peak in the Rainbow Calibration Particles (6 Peaks), 6.0-6.4μm (Mfr. No. 5556288)
• Approximate the background fluorescence of unstained cells

Specifications

• For Use With (Application): Flow Cytometry Calibration and Alignment
• Concentration: 107 particles/mL
• Diameter (Metric): 3 to 3.4 μm
• Volume (Metric): 5 mL
• For Use With (Equipment): Flow cytometer
• Format: Liquid

• Description: Rainbow Fluorescent Particles (Mid-range FL1 Fluorescence)
• Certifications/Compliance: 29 CFR 1910.1200, 91/155/EEC, 93/112/EC, RUO
• Content And Storage: Aqueous solution containing 0.01%NP40 and ≤0.02% sodium azide
• Color: Clear
• Includes: Single population of rainbow particles that are dyed to a single fluorescent intensity
• Material: Polystyrene