Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer I

Chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation

Supplier:  Applied Biosystems™ N8080244

Catalog No. N8080244

Description

Hot-start activation
The modified enzyme is provided in an inactive state. Upon thermal activation, the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.

Higher specificity, higher yield
AmpliTaq Gold DNA Polymerase can be activated partially or completely in a pre-PCR heat step, or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold DNA Polymerase is a chemical hot-start enzyme, there is no worry of biological contamination.

AmpliTaq Gold 360 DNA Polymerase for superior performance
Also available is AmpliTaq Gold 360 DNA Polymerase for even better PCR performance. Compared to AmpliTaq Gold DNA Polymerase, AmpliTaq Gold 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates. Supplied 360 GC Enhancer works through challenging GC-rich templates.

• Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
• Time-released thermal activation improves sensitivity in low copy number amplifications
• Successful multiplex PCR saves time and reagents
• Includes GeneAmp 10X PCR Buffer I containing 15mM MgCl₂

Hot Start PCR, PCR, PCR and Real-Time PCR, Routine PCR

Order Info

Shipping condition: Dry Ice

Specifications

• Content And Storage: • 12 x 250 U AmpliTaq Gold DNA Polymerase
  • 12 x 1.5 mL 10X PCR Buffer I containing MgCl₂
  
  Store at –15 to –30°C.
• Format: Tube
• Polymerase: AmpliTaq Gold DNA Polymerase
• Exonuclease Activity: 5' - 3'
• Product Type: DNA Polymerase With Buffer I
• Shipping Condition: Dry Ice
• Fidelity (vs. Taq): 1 X
• No. of Reactions: 2400 Reactions
• Reaction Format: Separate Components
• Starting Material: DNA

• Detection Method: Primer-probe
• GC-Rich PCR Performance: Low
• Reaction Speed: Standard
• Product Line: AmpliTaq Gold™
• Quantity: 12 x 250 Units
• Concentration: 5 U/μL
• Hot Start: Built-In Hot Start
• Overhang: 3'-A
• Size (Final Product): 5 kb or less

For Research Use Only. Not for use in diagnostic procedures.