Applied Biosystems™ AmpliTaq Gold™ 360 Master Mix

AmpliTaq Gold™ 360 PCR Master Mix contains everything required for successful PCR amplification in one convenient package–all components are premixed and premeasured.

Supplier:  Applied Biosystems™ 4398886

Catalog No. 43-988-86

Includes: AmpliTaq Gold 360 Master Mix is supplied at 2X concentration and the 360 GC Enhancer. Typically, 1.5mL of the 360 GC Enhancer is supplied with each 5mL of master Mix.. Source: Recombinant, modified form of theThermus aquaticusDNA polymerase gene expressed in E. coli. All solutions are prepared in deionized, filter-sterilized water.

Description

AmpliTaq Gold™ 360 PCR Master Mix contains everything required for successful PCR amplification in one convenient package–all components are premixed and premeasured. AmpliTaq Gold™ 360 Master Mix was designed for 360° coverage of a full range of targets. The master mix is supplied at 2X the recommended usage concentration for easy dilution when adding template and primers. Designed for convenience, the AmpliTaq Gold™ 360 PCR Master Mix scales to various reaction volumes for greater application and format flexibility. The main ingredient of the master mix is AmpliTaq Gold™ 360 DNA Polymerase but it also contains the world-class 360 GC Enhancer which can be added optionally for high GC-content templates. With the wide template coverage, optimization of PCR reaction conditions, is virtually eliminated with the use of AmpliTaq Gold™ 360 Master Mix.

Key features:

• Optimized for the broadest range of targets—from everyday to challenging
• Unmatched sensitivity, specificity, and yield
• Robust amplification of GC-rich sequences with market-leading 360 GC Enhancer
• Achieves the highest-quality sequencing data
• Easy-to-use, premixed master mix

Optimized for easy and challenging targets

Challenging targets include AT-rich, GC-rich, primer-dimer–forming amplicons, homopolymer repeats, and amplicons that pose sequencing challenges. Amplicons that previously required specialized enzymes and reaction conditions can now be reproducibly amplified with a single reagent under standardized conditions (see figure). Competitive benchmarking across more than 40 amplicons distinguishes AmpliTaq Gold™ 360 as the best-performing enzyme, ensuring the highest probability of success for the amplification of both everyday and challenging targets (see table).

Optimized for automated, hot-start PCR

AmpliTaq Gold™ 360 DNA Polymerase is the key ingredient in an automated, convenient, and efficient hot-start PCR. When AmpliTaq Gold™ 360 PCR Master Mix is added to the reaction mixture at room temperature, the inactive enzyme is not capable of primer extension. Any low-stringency mispriming events that may have occurred will not be enzymatically extended and subsequently amplified. An initial thermal incubation step is required for activation and ensures that active enzyme is generated only at temperatures where the DNA is fully denatured. The amount of AmpliTaq Gold™ 360 DNA polymerase increases in the reaction slowly with each cycle number, and specific product yield increases without buildup of nonspecific products, including primer dimers. Excellent specificity across a broad range of targets (see figure). The extreme specificity allows easier multiplexing and allelic discrimination.

Amplify low-copy amplicons and long targets

AmpliTaq Gold™ 360 DNA Polymerase efficiently amplifies targets present at low copy number (see figure), even in the presence of high concentrations of complex DNA, making it especially suited for low-copy pathogen detection, and amplification of targets from degraded DNA samples. The extreme purity of the enzyme contributes to its unmatched sensitivity. AmpliTaq Gold™ 360 DNA Polymerase efficiently and reproducibly amplifies long (up to 5 kb) sequences. The figure shown demonstrates robust PCR amplification of long human and plasmid DNA.

Order Info

Approved for shipment at Room Temperature or on Wet Ice

Specifications

• Content And Storage: • 2X AmpliTaq Gold 360 Master Mix, 1 x 50 mL
  • 360 GC Enhancer, 1 x 10 mL
  
  Store at -20°C until first use, then store at 4°C.
• GC-Rich PCR Performance: High
• Reaction Speed: Standard
• Green Features: Sustainable packaging
• Quantity: 50 mL
• For Use With (Application): Cloning, Hot-start PCR
• Fidelity (vs. Taq): 1X
• No. of Reactions: 2000 Reactions
• Reaction Format: SuperMix or Master Mix
• Starting Material: DNA

• Format: Tube
• Polymerase: AmpliTaq Gold 360 DNA Polymerase
• Exonuclease Activity: 5' - 3'
• Product Type: PCR Master Mix
• Shipping Condition: Room Temp or Wet Ice
• Concentration: 2X
• Hot Start: Built-In Hot Start
• Overhang: 3'-A
• Size (Final Product): 5 kb or less
• Volume: 50 mL

For Research Use Only. Not for use in diagnostic procedures.