Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl₂

Chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation

Catalog Number	Mfr. No.	Quantity
N8080249	Applied Biosystems™ N8080249	5000 Units
N8080259	Applied Biosystems™ N8080259	25 x 1000 Units
N8080241	Applied Biosystems™ N8080241	250 Units
N8080247	Applied Biosystems™ N8080247	1000 Units
N8080245	Applied Biosystems™ N8080245	3000 Units

Description

Hot-start activation
The modified enzyme is provided in an inactive state. Upon thermal activation, the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.

Higher specificity, higher yield
AmpliTaq Gold DNA Polymerase can be activated partially or completely in a pre-PCR heat step, or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold DNA Polymerase is a chemical hot-start enzyme, there is no worry of biological contamination.

AmpliTaq Gold 360 DNA Polymerase for superior performance
Also available is AmpliTaq Gold 360 DNA Polymerase for even better PCR performance. Compared to AmpliTaq Gold DNA Polymerase, AmpliTaq Gold 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates. Supplied 360 GC Enhancer works through challenging GC-rich templates.

• Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
• Time-released thermal activation improves sensitivity in low copy number amplifications
• Successful multiplex PCR saves time and reagents
• Includes GeneAmp 10X PCR Buffer II with MgCl₂

Hot Start PCR, Multiplex PCR, PCR, PCR and Real-Time PCR, Routine PCR

Order Info

Shipping condition: Dry Ice

Specifications

• Product Type: DNA Polymerase
• Concentration: 5 U/μL
• Overhang: 3'-A

For Research Use Only. Not for use in diagnostic procedures.