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Cytiva Amersham™ Megaprime™ DNA Labeling System

For labeling DNA probes to specific activities >1 x 109dpm/μg in 10 minutes

Supplier:  Cytiva RPN1606

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Catalog No. 45-000-809

Item Discontinued This item has been discontinued by the supplier. Please to view product availability in your area.


Includes: Primer solution; Labeling buffer; Reaction buffer; Enzyme; Standard DNA solution; Carrier DNA solution

Description

Description

Megaprime™ DNA Labeling Systems are used for rapid, random-prime labeling from as little as 25ng substrate DNA.

  • Features cloned Klenow fragment and random nonamers in the random-prime labeling technique
  • No need to remove unincorporated nucleotides before hybridization
  • Protocols for labeling directly from low melting point agarose

Specifications

Specifications

-15°C to -30°C
Primer solution 150μL, Labeling Buffer dATP, dGTP and dTTP in Tris HCl pH7.5, 2-mercaptoethanol and MgCl2 300μL, Nucleotide solutions dATP, cCTP, dGTP, dTTP in Tris HCl pH 8, 0.5mM EDTA, Reaction Buffer A 10 x concentra
Megaprime DNA Labeling System, dCTP (for use with labeled dCTP)
Membrane hybridization
30
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