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Cytiva Amersham™ ECF™ Substrate for Western Blotting

Used for detection of protein blots with fluorescence scanning equipment.  Cytiva Life Sciences™ Amersham™ ECF™ Substrate for Western Blotting uses a fluorescent substrate for alkaline phosphatase-based detection of protein blots.

Supplier:  Cytiva RPN5785

Catalog No. 45-000-947

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Includes: ECF Substrate, 60mL; Dilution buffer



Alkaline phosphatase dephosphorylates ECF Substrate to produce a fluorescent product, which localizes at the site of catalysis. Product fluoresces at 540 to 560nm when excited at the appropriate wavelength.

  • For use with fluorescence scanning equipment
  • Sensitivity comparable to chemiluminescence
  • Wide dynamic range of fluorescence scanning systems compared with film allows visualization and quantitation of weak and dark bands in the same scan
  • Eliminate need for darkroom and film

Western blotting, dot, and slot blotting



Fluorescence scanning equipment
ECF Substrate, 60mL, Dilution Buffer
Sufficient reagents for detecting 2500cm2 membrane


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