BL21 is the most widely used host background and has the advantage of being deficient in both lon (1) and ompT proteases. The designation (DE3) indicates that the strain is a lysogen of ?DE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter.
- Cells dispensed in 20μL volumes in 96 well polypropylene plate compatible with thermal cyclers and water baths
- Wells are individually sealed and have raised rims to prevent cross-contamination
- Seals can be easily pierced with standard pipet tips or removed for access
- Strips of caps are also provided for reliable sealing during manipulation and storage
- Groups of 24 wells can be easily split from the plate for processing smaller numbers of samples
- Efficiency: >2 × 106CFU/μg
|> 2 x 106CFU/μg
|Store HT96 Competent Cells, SOC Medium and Test Plasmid at -70°C or below. Store Cap Strips, Plate Lids and Reagent Reservoirs at room temperature
|For high-throughput transformation
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