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MilliporeSigma™ Novagen™ HT96™ BL21(DE3) Competent Cells

High-efficiency competent cells predispensed in 96-well plate for high-throughput applications

Supplier:  MilliporeSigma™ 710123

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Catalog No. 71-012-3MI

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Includes: HT96 BL21 (DE3) Competent Cells (1 or 4 plates), SOC Medium (1 × 14mL or 4 × 14mL), Test Plasmid (1 × 10μL or 2 × 10 μL), 8-Cap Strips (1 or 4), Reagent Reservoirs (1 or 4), Plate Lids (1 or 4)



BL21 is the most widely used host background and has the advantage of being deficient in both lon (1) and ompT proteases. The designation (DE3) indicates that the strain is a lysogen of ?DE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter.

  • Cells dispensed in 20μL volumes in 96 well polypropylene plate compatible with thermal cyclers and water baths
  • Wells are individually sealed and have raised rims to prevent cross-contamination
  • Seals can be easily pierced with standard pipet tips or removed for access
  • Strips of caps are also provided for reliable sealing during manipulation and storage
  • Groups of 24 wells can be easily split from the plate for processing smaller numbers of samples
  • Efficiency: >2 × 106CFU/μg



> 2 x 106CFU/μg
Store HT96 Competent Cells, SOC Medium and Test Plasmid at -70°C or below. Store Cap Strips, Plate Lids and Reagent Reservoirs at room temperature
For high-throughput transformation
1 Plate


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