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Thermo Scientific™ 1-Step Human High-Yield IVT Kit
Express functional proteins with 10 to 100 times greater yield per mL than other mammalian IVT systems with Thermo Scientific™ 1-Step Human High-Yield In Vitro Translation (IVT) Kits.
$884.00 - $5391.00
Catalog Number | Mfr. No. | Quantity | Type | Price | Quantity & Availability | ||||
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Catalog Number | Mfr. No. | Quantity | Type | Price | Quantity & Availability | ||||
PI88890
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Thermo Scientific™
88890 |
2 x 100 μL | 1-step Human High-yield Mini IVT Kit |
Each for $884.00
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PI88891
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Thermo Scientific™
88891 |
10 x 100 μL | 1-step Human High-yield Mini IVT Kit |
Each for $1,979.00
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PI88892
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Thermo Scientific™
88892 |
2 x 2 mL | 1-step Human High-yield Maxi IVT Kit |
Each for $5,391.00
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Description
The Thermo Scientific™ 1-Step Human High-Yield In Vitro Translation (IVT) Kits with continuous-feed device enables the expression of functional proteins with 10 to 100 times greater yield per mL than other eukaryotic IVT translation systems.
The 1-Step High-Yield IVT System uses modified HeLa cell extracts to take advantage of the robust human transcription and translation machinery and generate functional full-length proteins. In this system, protein expression is performed in a proprietary mini dialysis device that allows a continuous supply of nucleotides, amino acids and energy generating substrates into the reaction while removing inhibitors of proteins synthesis. This continuous-exchange cell-free (CECF) system enables protein expression for up to 24 hours and produces protein yields ranging from 250 to 750μg/mL. The complete kits come with all components required for proper transcription and translation of recombinant protein, including an optimized expression vector.Highlights:
High expression – up to 750μg/mL of expressed protein
Reproducibility – low variability between experiments
Fast – express high levels of protein with an overnight incubation
Functional – obtain more active protein than with bacterial protein expression systems
1-Step Protein Expression:
Streamlined reaction – requires only five components
Easy-to-use – transcription and translation is performed in one reaction step
Adapt for labeling – amenable to incorporation of heavy or unnatural amino acids
Includes:
Complete kits contain cell lysate, reaction mix, accessory proteins, 5X dialysis buffer, control DNA, cloning vector, mini dialysis devices and centrifuge tubes
Requires:
30°C incubator or water bath
Recommended for:
Production of recombinant proteins for structural analysis; Generate active proteins for protein interaction studies; Perform rapid expression and characterization of mutant proteins; Small-scale production of virual particles; Incorporation of unnatural amino acids or toxic protein production
Specifications
Cell-Free | |
Insoluble Protein |
For Research Use Only. Not for use in diagnostic procedures.
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