Test
Thermo Scientific™ Fermentas T3 RNA Polymerase is a DNA-dependent RNA polymerase with strict specificity for its double-stranded promoter.
Thermo Scientific™ T7 RNA Polymerase is a DNA-dependent RNA polymerase with strict specificity for its double-stranded promoter.
High-purity enzyme that's a mix of cloned RNA polymerase and RNase inhibitor
Thermo Scientific™ T7 RNA Polymerase is a DNA-dependent RNA polymerase with strict specificity for its double-stranded promoter.
Polymerase | T7 RNA Polymerase |
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Concentration | 20U/µL |
Catalyzes the addition of adenosine to the 3' end of RNA in a sequence-independent fashion
Thermo Scientific™ Fermentas T3 RNA Polymerase is a DNA-dependent RNA polymerase with strict specificity for its double-stranded promoter.
Thermo Scientific™ SP6 RNA Polymerase is a DNA-dependent RNA polymerase with strict specificity for its double-stranded promoter.
DNA-dependent RNA polymerase with high specificity for bacteriophage T7 promoter sequences
Promoter | T7 |
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Polymerase | T7 RNA Polymerase |
A cloned high-purity RNA polymerase with a high specificity for its promoter
Thermo Scientific™ SP6 RNA Polymerase is a DNA-dependent RNA polymerase with strict specificity for its double-stranded promoter.
Rabbit Polyclonal Antibody
Content And Storage | 4° C |
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Target Species | Human,Mouse |
Host Species | Rabbit |
Conjugate | Unconjugated |
Applications | Western Blot |
Form | Liquid |
Isotype | IgG |
Gene Accession No. | P08775, P24928 |
Antigen | RNA Polymerase II |
Regulatory Status | RUO |
Purification Method | Antigen affinity chromatography |
Gene Alias | RNA POL2 |
Gene | POLR2A |
Product Type | Antibody |
Gene ID (Entrez) | 20020, 5430 |
Formulation | TBS with 0.1% BSA and 0.09% sodium azide |
Immunogen | C-terminal repeat |
Classification | Polyclonal |
Primary or Secondary | Primary |
T7 RNA Polymerase Assay Kit Plus (enzyme included)
NxGen T7 RNA PolymeraseReliable and robust enzyme for production of mRNA transcripts in a variety of applications.Supplied at 50,000 U/mL in 50 mM Tris-HCl, 100 mM NaCl, 1 mM dithiothreitol, 1 mM EDTA, 50% glycerol, 0.1% Triton X-100, pH 7.9 @ 25°C. Also provided is 10X T7 RNA Polymerase Buffer which in 1X form is composed of 40 mM Tris-HCl, 6 mM MgCl2, 10 mM Dithiothreitol, 2 mM Spermidine, pH 7.9 @ 25°C.
T7 RNA Polymerase (High Concentration) is offered at a 20-fold higher concentration than our standard T7 RNA Polymerase and is ideal for experienced users interested in building and optimizing their own in vitro transcription reactions. The enzyme is accompanied by RNA Polymerase Reaction Buffer and magnesium chloride solution to further enable testing of different conditions such as nucleotide concentrations. For standard RNA synthesis and high yield reactions, we recommend our T7 RNA Polymerase and HiScribe kits. The HiScribe kits are specifically designed to produce high quality, high yield RNA in a short time with minimal optimization. In vitro transcription involves multiple components in addition to T7 RNA Polymerase including ribonucleotides, inorganic pyrophosphatase and RNase Inhibitor, which can be purchased separately. Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase that is highly specific for the T7 phage promoters.
Poly(A) Polymerase catalyzes the template independent addition of AMP from ATP to the 3 end of RNA.