Test
Used for the optimization of transfection and verification of editing efficiency in transfected cells.
Ambion™ Carrier RNA (1 mg/mL) is a component of all the MagMAX™ Isolation Kits that may be purchased separately.
Non-targeting gRNA sequences that do not recognize any sequence in the human or mouse genome.
Superior quality GeneArt CRISPR Nuclease mRNA is formulated to improve product performance.
Ready to use RNase-free solution with low toxicity to use with live cells for determination of RNA structure
Provides an efficient target for antiviral agents.
Ready-to-use, synthetic gRNAs maximizes and simplifies the performance of genome editing experiments.
Used for the optimization of transfection and verification of editing efficiency in transfected cells.
Used for the optimization of transfection and verification of editing efficiency in transfected cells.
STD SENSITVITY RNA KT 500SAMPL ..
NEN brand fluorescent nucleotide analogs are biologically active with a variety of DNA and/or RNA polymerases. Labeling methods such as: nick translation, random priming, polymerase chain reaction, 3'-end labeling, or transcription of RNA using SP6, T3, or T7 RNA polymerases may be used. Some analogs demonstrate variations in relative performance depending upon nucleotide and fluorophore selected due to enzyme preferences. Labeled probes may be used in applications including (but not limited to) microarrays and chromosome mapping. These analogs are intended to be detected directly by their fluorescence properties.
The 5' terminal m7G cap present on most eukaryotic mRNAs promotes translation in vitro at the initiation level. For most RNAs, elimination of the cap structure causes a loss of stability, especially against exonuclease degradation, and a decrease in the formation of the initiation complex of mRNAs for protein synthesis. Certain prokaryotic mRNAs containing a 5' terminal cap structure are translated as efficiently as or more efficiently than eukaryotic mRNAs in a eukaryotic cell-free protein synthesizing system. Also a cap requirement has been observed for splicing eukaryotic substrate RNAs. A method using E. coli RNA Polymerase primed with m7G(5' )ppp(5' )G or m7G(5' )ppp(5' )A for an efficient in vitro synthesis of capped RNAs has been developed by Contreas. Larger amounts of capped RNAs are produced by transcription systems using SP6 RNA polymerase primed with m7G(5' )ppp(5' )G).
CF Dye Succinimidyl Esters (SE, also known as NHS esters) are amine-reactive fluorescent dyes. SE dyes are commonly used to label antibodies and other proteins on free amine groups, such as lysine residues. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Orange fluorescent CF532 dye has excitation/emission at 527/558 nm.
CF405L MALEIMIDE 1 UMOL
NEBNextMultiplex Oligos for Illumina(Unique Dual Index UMI Adaptors DNA Set 2) are built for more streamlined and informative NGS multiplexing, tracking the sequencing reaction at the individual molecule level and enabling bioinformatic removal of misassigned reads for optimal accuracy. These index adaptors contain a pair of unique dual indices (i5 and i7) and one UMI sequence, making the resulting library multiplex-ready, with or without PCR. This set includes 96 unique dual index UMI adaptors, packaged in a single-use 96-well plate with a pierceable foil seal. The universal primer mix is supplied in a separate vial. Combining UMI Adaptors DNA Set 2 with one of the NEBNext DNA library prep kits is simple and straightforward if you follow the protocols found in the library prep kit manuals. With four sets of NEBNext Unique Dual Index UMI Adaptors available, you can multiplex up to 384 samples. Sample sheets for multiplexing are available.