MilliporeSigma Western Blotting Solutions

MilliporeSigma Western Blotting Solutions

Designed to Help You Work More Efficiently

Get consistent and accurate results with a comprehensive range of MilliporeSigma products for western blotting:

  • Immobilon GO, the new walk-away immunodetection device
  • The SNAP I.d.™ System, a fast detection platform
  • Immobilon™-P membrane, the most referenced PVDF membrane on the market 
  • Immobilon-E, the first pre-wet PVDF membrane without alcohol
  • Plus detection reagents that are simple to use

Immobilon NOW Transfer Membrane Video

See how to get perfectly sized western blotting membrane every time with Immobilon NOW Transfer Membranes.

Request a Protein Blotting Handbook

Get valuable tips and advice to help improve your western blotting from this handbook. Just fill out the form to request a copy today.

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Immobilon™-E Transfer Membrane

Immobilon™-E Transfer Membrane

Available in a roll or easy-to-use precut sheets that fit standard mini or midi blots, these membranes eliminate the need for alcohol pre-wetting.

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Immobilon-E Blotting Sandwiches

Immobilon-E Blotting Sandwiches

A convenient way to process multiple western blots for standard tank transfers. Each sandwich has Immobilon-E membranes interleaved with sheets of blotting paper.

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Immobilon-NOW Transfer Membranes

Immobilon-NOW Transfer Membranes

Compatible with mini or midi gels, these membranes offer the convenience of pre-cut sheets with the flexibility of membrane rolls.

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Immobilon Transfer Membrane Half Rolls

Immobilon Transfer Membrane Half Rolls

Save money and custom-cut your Immobilon-E, -FL, -P, or ‑PSQ transfer membranes from these convenient half rolls.

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Immobilon Signal Enhancer for Immunodetection

Immobilon Signal Enhancer for Immunodetection

Combines signal amplification and blocking in a ready-to-use reagent that amplifies low signal intensity without amplifying the noise.

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Immobilon Block Noise Canceling Reagents

Immobilon Block Noise Canceling Reagents

Minimize background noise for easier detection of even low-abundance proteins with these protein-free blocking reagents.

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ReBlot™ Antibody Stripping Solution

ReBlot™ Antibody Stripping Solution

Now you can re-use your western blots ─ strip just the antibodies from blots at room temperature in approximately 15 minutes.

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Immobilon Western Chemiluminescent HRP Substrate

ImmobilonWestern Chemiluminescent HRP Substrate

Combining high sensitivity and low background, this substrate provides high sensitivity over a broad detection range.

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Immobilon ECL Ultra Western HRP Substrate

ImmobilonECL Ultra Western HRP Substrate

This ultra-sensitive reagent has a long signal duration for detecting horseradish peroxidase (HRP)-labeled antibodies on western blots.

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Immobilon Classico Western HRP Substrate

ImmobilonClassico Western HRP Substrate

A premixed, ready-to-use reagent for the chemiluminescent detection of high- to medium-abundance proteins in western blots that use HRP-conjugated antibodies.

100mL  500mL


Immobilon Crescendo Western HRP Substrate

ImmobilonCrescendo Western HRP Substrate

A premixed, ready-to-use reagent for the chemiluminescent detection of medium- to low-abundance proteins in western blots that use HRP-conjugated antibodies.

100mL   500mL

Immobilon Forte Western HRP Substrate

ImmobilonForte Western HRP Substrate

Detect medium- to low-abundance proteins in western blots that use HRP-conjugated antibodies with this premixed detection reagent that’s stable at room temperature and more sensitive.

100mL   500mL


SNAP i.d.™ 2.0 Protein Detection System

SNAP i.d.™ 2.0 Protein Detection System

Uses a vacuum to actively drive antibodies and buffers right through the membrane to shorten the time for blocking, washing, and antibody incubations to just 30 minutes.

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Immobilon-P PVDF Transfer Membranes

Immobilon-P PVDF Transfer Membranes

Optimized for binding proteins transferred from a variety of gel matrices with superior handling, staining, and resistance.

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