MilliporeSigma Western Blotting Solutions
Designed to Help You Work More Efficiently
Get consistent and accurate results with a comprehensive range of MilliporeSigma products for western blotting:
- Immobilon GO, the new walk-away immunodetection device
- The SNAP I.d.™ System, a fast detection platform
- Immobilon™-P membrane, the most referenced PVDF membrane on the market
- Immobilon-E, the first prewet PVDF membrane without alcohol
- Plus detection reagents that are simple to use
Immobilon NOW Transfer Membrane Video
See how to get perfectly sized western blotting membrane every time with Immobilon NOW Transfer Membranes.
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Immobilon™-E Transfer Membrane
Available in a roll or easy-to-use precut sheets that fit standard mini or midi blots, these membranes eliminate the need for alcohol pre-wetting.
Immobilon-E Blotting Sandwiches
A convenient way to process multiple western blots for standard tank transfers. Each sandwich has Immobilon-E membranes interleaved with sheets of blotting paper.
Immobilon-NOW Transfer Membranes
Compatible with mini or midi gels, these membranes offer the convenience of pre-cut sheets with the flexibility of membrane rolls.
Immobilon Transfer Membrane Half Rolls
Save money and custom-cut your Immobilon-E, -FL, -P, or ‑PSQ transfer membranes from these convenient half rolls.
Immobilon Signal Enhancer for Immunodetection
Combines signal amplification and blocking in a ready-to-use reagent that amplifies low signal intensity without amplifying the noise.
Immobilon Block Noise Canceling Reagents
Minimize background noise for easier detection of even low-abundance proteins with these protein-free blocking reagents.
ReBlot™ Antibody Stripping Solution
Now you can re-use your western blots ─ strip just the antibodies from blots at room temperature in approximately 15 minutes.
ImmobilonWestern Chemiluminescent HRP Substrate
Combining high sensitivity and low background, this substrate provides high sensitivity over a broad detection range.
ImmobilonECL Ultra Western HRP Substrate
This ultra-sensitive reagent has a long signal duration for detecting horseradish peroxidase (HRP)-labeled antibodies on western blots.
SNAP i.d.™ 2.0 Protein Detection System
Uses a vacuum to actively drive antibodies and buffers right through the membrane to shorten the time for blocking, washing, and antibody incubations to just 30 minutes.
Immobilon GO Immunodetection Device
Apply the principles of lateral flow assays to western blots for walk-away processing in three easy steps and results in as little as three hours.
Immobilon-P PVDF Transfer Membranes
Optimized for binding proteins transferred from a variety of gel matrices with superior handling, staining, and resistance.