Respiratory Tract Microbiota Detection


Detection of exposure of viral, bacterial, and fungal microbes in the upper and lower respiratory tract is crucial because if undetected and, they could cause serious diseases. Traditional methods of microbiota detection include immunoassays, which have limitations, and culture-based methods that are easily contaminated and often overlook the presence of bacteria and fungi. Molecular testing is an alternative option, but it is expensive and lacks flexibility. Also, these methods focus primarily on detection of either viruses or bacteria, but not both.

With Applied Biosystems™ OpenArray™ and TaqMan® testing solutions, you get superior microbiota detection that includes coverage of 42 verified pathogen targets or customizable options. When processed through Applied Biosystems™ QuantStudio™ Real-Time PCR Systems, the sensitive, flexible-content, syndromic panels enable reliable results. OpenArray and TaqMan Array Card technology is also cost effective and works with multiple sample types.

Contact your Fisher Healthcare product manager to learn more.

Test Features

  • Unparalleled Coverage: With 42 verified pathogen targets, Applied Biosystems panels feature the most pathogenic markers of any test on the market.
  • Flexible: Verified for TaqMan Array Cards and OpenArray plates and offer customized solutions for 96- and 384-well plates or single-tube assays.
  • Affordable: Up to three times more cost effective than other commercially available panel-based molecular respiratory tests.
  • Versatile: Accepts multiple sample types, including nasopharyngeal swabs, bronchoalveolar lavage (BAL), and nasopharyngeal aspirate.

TaqMan Assays Included

  • TaqMan Array Card: Respiratory Tract Microbiota Comprehensive Card
  • OpenArray: Respiratory Tract Microbiota Plate
  • TaqMan PreAmp Pool, Respiratory Tract Microbiota

Controls Included

  • TaqMan Respiratory Tract Microbiota Amplification Control
  • TaqMan Universal RNA Spike-In/Reverse Transcription (Xeno) Control
  • TaqMan Universal Extraction Control Organism

Data and Support

  • Application Note: Detecting Respiratory Tract Microbiota Using TaqMan Array Cards

TaqMan Array Card Format

Number of Wells384
Number of Targets42 Respiratory Plus 4 Control (Xeno, RNase P, and Bacillus atropheus)
Number of Samples8
Master Mix Volume400 μL Per TaqMan Array Card (384 Reactions)
Reaction Volume1 μL
Samples/DayUp to 64
Compatible InstrumentsQuantStudio 12K Flex Real-Time PCR System with TaqMan Array Card Block
Time to Results5 hours

OpenArray Plate Format

Number of Wells2,688
Number of Targets31 Respiratory Plus 4 Control (Xeno, RNase P, and Bacillus atropheus)
Number of SamplesUp to 24 Samples Per Plate (Spotted in Triplicate)
Master Mix Volume120 μL Per OpenArray Plate (3,072 Reactions)
Reaction Volume33 nL
Samples/DayUp to 288
Compatible InstrumentsQuantStudio 12K Flex Real-Time PCR System with OpenArray Block
Time to Results5 hours

Microbiota Targets

Included with TaqMan Array Card. Those included with OpenArray Plate are marked with an asterisk (*).

Pathogen TypeTarget Pathogen
BacterialBordetella (PAN)*
Citrobacter freundii
Bordetella holmesii
Bordetella pertussis*
Chlamydophila pneumoniae*
Mycoplasma pneumoniae*
Streptococcus pneumoniae*
Coxiella burnetii
Staphylococcus aureus
Klebsiella pneumoniae*
Legionella pneumophila*
Moraxella catarrhalis
Haemophilus influenzae*
FungalPneumocystis jirovecii
Pathogen TypeTarget Pathogen
Coronavirus HKU1*
Coronavirus NL63*
Coronavirus 229E*
Coronavirus OC43*
Human Metapneumovirus*
Enterovirus D68
Influenza A (Pan)*
Influenza A/H1-2009*
Influenza A/H3*
Influenza B*
Parainfluenza 1*
Parainfluenza 2*
Parainfluenza 3*
Parainfluenza 4*
Respiratory Syncytial Virus A*
Respiratory Syncytial Virus B*
Epstein-Barr Virus (EBV)*
Human Herpesvirus 6 (HHV-6)*
Varicella Zoster Virus (VZV)*

For Research Use Only. Not for use in diagnostic procedures.