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PureCol EZ Gel is a 5 mg/ml ready-to-use bovine atelocollagen solution that forms a 3D hydrogel by simply warming to 37°C. This product is pH neutral and isotonic formulated with DMEM/F12 medium and ready for immediate cell addition and 3D hydrogels. The product consists of purified Type I collagen at a concentration of 0.5%, (~5 mg/ml), DMEM/F-12 medium and a mixture of L-glutamine and dipeptide (L-alanine-L-glutamine) to provide a long-lasting L-glutamine source for cell culture. PureCol EZ Gel is designed to improve gel consistency by pre-formulation of the medium and adjustment of the product to a neutral pH. This product avoids the inconsistencies in the preparation of the gel that can arise through variables of reagent addition, pH adjustment and handling conditions. PureCol EZ Gel is ideal for providing a firm gel and can also be used in the preparation of a thin layer for culturing cells. This product is sterile. Collagen Training Video: https://youtu.be/YcK2vUpNt0g