Bead Based Assays
Bead Based Assays
Revvity Health Sciences Inc Anti-human IgG (Fc specific) AlphaLISA Acceptor Beads 5mg
These beads can be used in conjunction with Alpha Donor beads to create AlphaLISA no-wash immunoassays for: Analyte detection assays Biomarker detection assays Antibody detection assays Antibody binding studies Protein-protein interaction assays Other immunoassays In a typical AlphaLISA assay, 1 mg of Acceptor beads is sufficient to run 1,000-2,000 wells using a 50 µL reaction volume. Features: No-wash steps, no separation steps Ease-of-use: few addition steps, fast assay development Broad range of affinities: detect strong or weak interactions, from pM to mM affinity Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders AlphaScreen and AlphaLISA are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym Alpha stands for amplified luminescent
