PCR Cloning Kits
PCR Cloning Kits
Thermo Scientific™ Maxima H Minus Double-Stranded cDNA Synthesis Kit
The Thermo Scientific Maxima H Minus Double-Stranded cDNA Synthesis Kit is a complete system for efficient synthesis of double-stranded cDNA from total RNA or mRNA.
Invitrogen™ Ambion™ T4 RNA Ligase, cloned, 5 U/µL
Catalyzes formation of a phosphodiester linkage between a 5′-phosphoryl-terminated nucleic acid donor and a 3′-hydroxyl-terminated nucleic acid acceptor
Invitrogen™ RNase T1 (1 U/μL)
Optimized for researchers performing RNA structure, RNA sequencing, protein footprinting, and boundary experiments. Invitrogen™ Ambion™ RNase T1 (1U/μL) includes RNase T1, 10X RNA Structure Buffer, 1X Alkaline Hydrolysis Buffer, Yeast RNA, Precipitation/Inactivation Buffer and Gel Loading Buffer II.
Invitrogen™ T7 RNA Polymerase-Plus™ Enzyme Mix (200 U/μL)
High-purity enzyme that's a mix of cloned RNA polymerase and RNase inhibitor
Invitrogen™ TOPO™ XL-2 Complete PCR Cloning Kit, with One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli Cells
Provides all the necessary elements for efficient cloning of extra-long PCR products (up to 13 kb)
Invitrogen™ Ambion™ SP6 RNA Polymerase, cloned, 20 U/µL
A cloned high-purity RNA polymerase with a high specificity for its promoter
Invitrogen™ Poly(A) Polymerase (cloned) 2 U/μL
Catalyzes the addition of adenosine to the 3' end of RNA in a sequence-independent fashion
Invitrogen™ Second Strand cDNA Synthesis Kit
Invitrogen™ Second Strand cDNA Synthesis Kit is design to produce double-stranded cDNA using first strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.
Clontech Labs 3P In-Fusion HD EcoDry™ Cloning Kit, 96 Rxns
The In-Fusion HD EcoDry Cloning Kit enables high-efficiency, high-fidelity, directional cloning of one or more PCR fragments into any vector. This package includes a 96-well plate of In-Fusion HD EcoDry Mix, linearized pUC19 control vector, and a 2-kb control insert.
Clontech Labs 3P In-Fusion Snap Assembly Starter Bundle | 10 Rxns
In-Fusion Snap Assembly Master Mix enables high-efficiency, high-fidelity, directional cloning of one or more PCR fragments into any vector.
Clontech Labs 3P In-Fusion Snap Assembly Value Bundle | 50 rxns
In-Fusion Snap Assembly Master Mix enables high-efficiency, high-fidelity, directional cloning of one or more PCR fragments into any vector. In addition to the cloning kit, this package includes a NucleoSpin Gell and PCR Clean-Up Kit, Stellar Competent Cells, and PrimeSTAR Max DNA Polymerase.
Clontech Labs 3P In-Fusion Snap Assembly Master Mix | 250 Rxns
In-Fusion Snap Assembly Master Mix is designed for fast, directional cloning of one or more fragments of DNA into any vector. This proprietary master mix fuses DNA fragments (e.g., PCR-generated sequences and linearized vectors) efficiently and precisely by recognizing a 15-bp overlap at their ends. This 15-bp overlap can be engineered into the primers designed for PCR amplification of the desired sequences. In Fusion Snap Assembly Master Mix offers high efficiency, even for applications that can be challenging, including the cloning of long fragments, short oligonucleotides, and multiple fragments.