PCR Cloning Kits
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PCR Cloning Kits
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Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ TOP10 Chemically Competent E. coli
Provides a quick, one-step cloning strategy for directly inserting a Taq-amplified PCR product into a plasmid vector. Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ TOP10 Chemically Competent E. coli is available in 20- and 40-reaction kit sizes.
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Promoter | T7 |
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Content And Storage | TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. The competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
Format | Kit |
Product Type | Cloning Kit |
Cloning Method | TA Cloning |
Bacterial or Yeast Strain | TOP10 |
For Use With (Application) | PCR Cloning |
Product Line | One Shot™ |
Cell Type | Chemically Competent E. coli |
Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector, without competent cells
Provides quick, one-step cloning strategy for directly inserting Taq-amplified PCR product into plasmid vector
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Promoter | T7 |
---|---|
Content And Storage | TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
Format | Kit |
Product Type | Cloning Kit |
Cloning Method | TA Cloning |
Bacterial or Yeast Strain | Not Included |
For Use With (Application) | PCR Cloning |
Vector | pCR2.1 |
Product Line | TA Cloning™ |
Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with One Shot™ TOP10 chemically competent E. coli cells
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for subcloning
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Content And Storage | Box 1: • Topoisomerase I-activated pCR™2.1-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C. |
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Format | Kit |
Product Type | Cloning Kit |
Cloning Method | TOPO™-TA |
Bacterial or Yeast Strain | TOP10 |
Cell Type | Chemically Competent |
Thermo Scientific™ CloneJET PCR Cloning Kit
Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.
Antibiotic Resistance Bacterial | Ampicillin (AmpR) |
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Format | Kit |
Sample Type | PCR Products |
Cloning Method | Restriction Enzyme⁄ MCS, Blunt PCR |
For Use With (Application) | PCR Cloning |
Vector | pJET1.2/blunt |
Product Line | CloneJET |
Invitrogen™ T7 RNA Polymerase-Plus™ Enzyme Mix (200 U/μL)
High-purity enzyme that's a mix of cloned RNA polymerase and RNase inhibitor
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Invitrogen™ pENTR™/D-TOPO™ Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli
Efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector for entry into Gateway System or MultiSite Gateway System
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Invitrogen™ Zero Blunt™ PCR Cloning Kit, without competent cells
Offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with proofreading, thermostable DNA polymerases
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Promoter | T7 |
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Content And Storage | Zero Blunt™ PCR cloning kits contain linearized pCR™-Blunt vector, ExpressLink™ T4 DNA ligase , 5X ExpressLink™ T4 DNA ligase buffer, control template, dNTPs, sterile water, and M13 forward and reverse primers. Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
Format | Kit |
Product Type | PCR Cloning Kit |
Cloning Method | Blunt PCR |
Bacterial or Yeast Strain | Not Included |
Vector | Blunt DNA Cloning Vectors |
Product Line | TOPO™, Zero Blunt™ |
Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, with One Shot™ TOP10 Chemically Competent E. coli
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of proofreading-polymerase–amplified blunt-end PCR products into a plasmid vector for sequencing
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Promoter | T7, T3 |
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Format | Kit |
Product Type | PCR Cloning Kit |
Cloning Method | Blunt TOPO™ |
Bacterial or Yeast Strain | TOP10 |
For Use With (Application) | Chromatin Biology |
Vector | Blunt DNA Cloning Vectors |
Product Line | One Shot™ |
Cell Type | Chemically Competent |
Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli cells
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Promoter | SP6 |
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Content And Storage | Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C. |
Format | Kit |
Product Type | PCR Cloning Kit |
Cloning Method | Blunt TOPO™ |
Bacterial or Yeast Strain | TOP10 |
Cell Type | Chemically Competent |
Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, without competent cells
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with One Shot™ MAX Efficiency™ DH5α-T1R E. coli
Provides a highly efficient, 5 minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Promoter | T7, T3 |
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Content And Storage | Box 1: • Topoisomerase I-activated pCR™4-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • T3, T7, M13 forward, and M13 reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C. |
Format | Kit |
Product Type | Cloning Kit |
Cloning Method | TOPO™-TA |
Bacterial or Yeast Strain | DH5α |
For Use With (Application) | Chromatin Biology |
Vector | TOPO-TA Cloning Vectors |
Product Line | One Shot™ |
Invitrogen™ Zero Blunt™ TOPO PCR Cloning Kit for Subcloning
Combines the unique Zero Background™ technology with the pCR™- Blunt II-TOPO vector to allow easy, high-efficiency cloning of blunt-end PCR products
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Invitrogen™ Bac-to-Bac™ N-His TOPO™ Cloning Kit
Bac-to-Bac™ N-His TOPO™ Cloning Kit
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Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli (Supply Center Packaging)
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector
![ENCOMPASS_PREFERRED](/content/dam/fishersci/glyphs/encompass_preferred.png)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, without competent cells
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Antibiotic Resistance Bacterial | Ampicillin (AmpR), Kanamycin (KanR) |
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Format | Kit |
Product Type | Cloning Kit |
Cloning Method | TOPO™-TA |
Product Line | TOPO™ |