Primary Antibodies
Primary Antibodies
Primary antibodies are immunoglobulins that recognize and bind to a specific antigen of interest with high affinity and specificity to purify, detect, and measure that antigen. Includes antibody pairs for specific biochemical applications.
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LAMP-2/CD107b Antibody (H4B4) - BSA Free, Novus Biologicals™
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Mouse Monoclonal Antibody has been used in 23 publications
Content And Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
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Target Species | Human,Mouse,Rat (Negative) |
Host Species | Mouse |
Conjugate | Unconjugated |
Applications | Western Blot,Immunoassay,ELISA,Immunohistochemistry,Immunohistochemistry,Immunofluorescence,Immunohistochemistry (Paraffin),Immunohistochemistry (Frozen),Immunohistochemistry (Frozen),CyTOF,CyTOF |
Form | Purified |
Isotype | IgG1 κ |
Gene Accession No. | P13473 |
Research Discipline | Cardiovascular Biology, Cellular Markers, Chaperone Mediated Autophagy (CMA), Cognition and Behavior, Growth and Development, Immunology, Innate Immunity, Lipid and Metabolism, Lysosome Markers, Mast Cell Markers, Membrane Trafficking and Chaperones |
Concentration | 1.0 mg/mL |
Antigen | LAMP-2/CD107b |
Gene Symbols | LAMP2 |
Regulatory Status | RUO |
Purification Method | Protein G purified |
Dilution | Western Blot 1:1000, Simple Western 1:25, Flow Cytometry 1 ug per million cells, ELISA, Immunohistochemistry 1:100 - 1:200, Immunocytochemistry/Immunofluorescence 1:10-1:100, Immunohistochemistry-Paraffin 1:100 - 1:200, Immunohistochemistry-Frozen 1:100 - 1:200, Immunoblotting reported in scientific literature (PMID 29643474), CyTOF-ready, Knockout Validated |
Molecular Weight of Antigen | 45 kDa |
Gene Alias | CD107 antigen-like family member B, CD107b, CD107b antigen, LAMP-2, LAMPB, LGP110, LGP-96, Lysosomal Associated Membrane Protein 2, lysosomal-associated membrane protein 2, lysosome-associated membrane glycoprotein 2, Lysosome-associated membrane protein |
Gene ID (Entrez) | 3920 |
Immunogen | LAMP-2/CD107b Antibody (H4B4) was made using a human adherent spleen cells. |
Classification | Monoclonal |
Primary or Secondary | Primary |
Test Specificity | Human. Does not react with Mouse or Rat. |
Clone | H4B4 |
NeuN Mouse anti-Avian, Chicken, Ferret, Human, Mouse, Pig, Rat, Salamander, Clone: A60, MilliporeSigma™
Mouse Monoclonal Antibody
ABCA4 Antibody (3F4), Novus Biologicals™
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Mouse Monoclonal Antibody has been used in 4 publications
AIF-1/Iba1 Antibody, Novus Biologicals™
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Goat Polyclonal Antibody has been used in 192 publications
Content And Storage | Store at -20C. Avoid freeze-thaw cycles. |
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Target Species | Human,Mouse,Rat,Canine,Feline |
Host Species | Goat |
Conjugate | Unconjugated |
Applications | Western Blot,Immunohistochemistry,Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Paraffin),Immunohistochemistry (Frozen),ELISA,Immunohistochemistry (Free Floating),Immunoassay |
Isotype | IgG |
Gene Accession No. | P55008 |
Research Discipline | Autoimmune Diseases, Cancer, Cell Biology, Cell Cycle and Replication, Immunology, Inflammation, Lipid and Metabolism, Microglia Markers, Neuroscience |
Concentration | 0.5 mg/mL |
Antigen | AIF-1/Iba1 |
Gene Symbols | AIF1 |
Regulatory Status | RUO |
Purification Method | Affinity Purified |
Gene Alias | AIF-1, allograft inflammatory factor 1, G1, IBA1, IbaI, interferon gamma responsive transcript, Ionized calcium-binding adapter molecule 1, IRT1, IRT-1, Protein G1 |
Gene ID (Entrez) | 199 |
Formulation | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA with 0.02% Sodium Azide |
Immunogen | This AIF-1/Iba1 Antibody was developed against a peptide with sequence C-TGPPAKKAISELP, from the C Terminus of the protein sequence according to NP_116573.1; NP_001614.3. |
Classification | Polyclonal |
Primary or Secondary | Primary |
Test Specificity | This AIF-1/Iba1 Antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3). AIF-1/Iba1 is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. |
CLEC9a Antibody (8F9) - Azide and BSA Free, Novus Biologicals™
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Mouse Monoclonal Antibody
CD24 Antibody (ML5) - Azide and BSA Free, Novus Biologicals™
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Mouse Monoclonal Antibody
Content And Storage | Store at 4°C. Do not freeze. |
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Conjugate | Unconjugated |
Target Species | Human |
Host Species | Mouse |
Applications | Flow Cytometry,Immunohistochemistry,Immunofluorescence,Immunohistochemistry (Frozen) |
Form | Purified |
Isotype | IgG2a κ |
Antigen | CD24 |
Regulatory Status | RUO |
Purification Method | Protein A or G purified |
Gene Alias | CD 24, CD24 antigen, CD24 antigen (small cell lung carcinoma cluster 4 antigen), CD24 molecule, CD24Asignal transducer CD24, FLJ22950, FLJ43543, MGC75043, Small cell lung carcinoma cluster 4 antigen |
Formulation | PBS |
Gene ID (Entrez) | 100133941 |
Classification | Monoclonal |
Immunogen | The immunogen was the full length version of the protein: CD24 (ML5) |
Primary or Secondary | Primary |
Clone | ML5 |
RBFOX3/NeuN Antibody, Novus Biologicals™
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Rabbit Polyclonal Antibody has been used in 2 publications
Content And Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
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Target Species | Human,Mouse,Rat |
Host Species | Rabbit |
Conjugate | Unconjugated |
Applications | Western Blot,Immunohistochemistry,Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen) |
Form | Antisera |
Isotype | IgG |
Gene Accession No. | A6NFN3 |
Research Discipline | DNA replication Transcription Translation and Splicing, Neuronal Cell Markers, Neuroscience |
Concentration | 1 mg/ml |
Antigen | RBFOX3/NeuN |
Gene Symbols | RBFOX3 |
Regulatory Status | RUO |
Purification Method | Affinity purified |
Dilution | Western Blot 1:5000-1:10000, Simple Western 1:500, Immunohistochemistry 1:500-1:1000, Immunocytochemistry/Immunofluorescence 1:500-1:1000, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating 1:500-1:1000 |
Molecular Weight of Antigen | 48 kDa |
Gene Alias | FLJ56884, FLJ58356, Fox-1 homolog C, FOX3, FOX-3, hexaribonucleotide binding protein 3, HRNBP3, NeuN, neuronal nuclei, RBFOX3, RNA binding protein fox-1 homolog 3, RNA binding protein, fox-1 homolog (C. elegans) 3 |
Gene ID (Entrez) | 146713 |
Formulation | 50% PBS, 50% glycerol with 5mM Sodium Azide |
Immunogen | The N-terminal 100 amino acids of human RBFOX3/NeuN as expressed in and purified from E. coli. [UniProt# A6NFN3] |
Classification | Polyclonal |
Primary or Secondary | Primary |
Test Specificity | Human, bovine, porcine, mouse, rat and other mammals. |
APP A4 Mouse anti-Canine, Human, Mouse, Monkey, Pig, Rat, Fish, Clone: 22C11, MilliporeSigma™
Mouse Monoclonal Antibody
KAP1, p Ser824 Antibody, Novus Biologicals™
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Rabbit Polyclonal Antibody has been used in 31 publication
p19ARF/CDKN2A Antibody (12-A1-1), Novus Biologicals™
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Rat Monoclonal Antibody has been used in 5 publications
Content And Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
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Target Species | Mouse,Hamster (Negative),Human (Negative) |
Host Species | Rat |
Conjugate | Unconjugated |
Applications | Western Blot,ELISA,Immunohistochemistry,Immunocytochemistry,Immunofluorescence,Immunoprecipitation,Immunohistochemistry (Paraffin) |
Form | Ascites |
Isotype | IgG2b |
Gene Accession No. | Q64364 |
Research Discipline | Cancer, Cell Biology, Cell Cycle and Replication, Chromatin Research, p53 Pathway, Transcription Factors and Regulators, Tumor Suppressors |
Antigen | p19ARF |
Gene Symbols | CDKN2A |
Regulatory Status | RUO |
Purification Method | Unpurified |
Dilution | Western Blot 1:500, ELISA 1:5000-1:10000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:100, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500 |
Molecular Weight of Antigen | 19 kDa |
Gene Alias | ARF, CDK4 inhibitor p16-INK4, CDK4IP14ARF, CDKN2, cell cycle negative regulator beta, CMM2P16-INK4A, Cyclin-dependent kinase 4 inhibitor A, cyclin-dependent kinase inhibitor 2A, cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4), INK4, INK4A, MLMP16INK4, MTS-1, MTS1P14, Multiple tumor suppressor 1, p14, p14ARF, p16, p16-INK4, p16INK4a, p16-INK4a, P19, p19ARF, TP16 |
Gene ID (Entrez) | 12578 |
Formulation | Ascites with 0.1% Sodium Azide |
Immunogen | A synthetic peptide made to a sequence (within residues 1-100) of mouse p19ARF. [UniProt# Q64364] |
Classification | Monoclonal |
Primary or Secondary | Primary |
Test Specificity | This antibody is specific for murine p19ARF. It does not recognize human p14Arf nor Golden hamster p13Arf. |
Clone | 12-A1-1 |